RRC ID 44150
著者 Miki Y, Hirano K, Beppu M.
タイトル Macrophage recognition of thiol-group oxidized cells: recognition of carbohydrate chains by macrophage surface nucleolin as apoptotic cells.
ジャーナル Biosci Biotechnol Biochem
Abstract The mechanism was investigated for macrophage recognition of cells oxidized by diamide, a thiol group-specific oxidizing reagent. Jurkat cells exposed to various concentrations of diamide were recognized by macrophages, the cells exposed to 25 µM diamide being best recognized. CD43, a major glycoprotein on the Jurkat cell surface, tended to form clusters upon diamide oxidization, and pretreating Jurkat cells with the anti-CD43 antibody inhibited macrophage binding. This indicates that macrophages appeared to recognize CD43. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and a Western blot analysis of CD43 of the diamide-oxidized cells showed no increase in the amount of cross-linked CD43 compared with control cells, indicating that cross-linking of CD43 by a disulphide bond was not involved in the clustering. Both CD43 clustering and binding of the oxidized cells to macrophages was prevented by the caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethyl ketone (Z-VAD-fmk), suggesting that the oxidized and macrophage-bound cells were undergoing apoptosis. A closer examination revealed that the caspase-3 activity, chromatin condensation, and DNA fragmentation in Jurkat cells were all increased by oxidation. The macrophage receptor involved in the binding appeared to be the cell-surface protein, nucleolin; an anti-nucleolin antibody treatment inhibited the binding. These results suggest that thiol group-oxidized cells underwent early apoptosis and were recognized by nucleolin on macrophages as early apoptotic cells.
巻・号 76(11)
ページ 2068-74
公開日 2012-1-1
DOI 10.1271/bbb.120413
PII DN/JST.JSTAGE/bbb/120413
PMID 23132587
MeSH Apoptosis* / drug effects Carbohydrate Metabolism* / drug effects Diamide / pharmacology Humans Jurkat Cells Ligands Macrophages / cytology* Macrophages / drug effects Macrophages / metabolism* Oxidation-Reduction / drug effects Phosphoproteins / metabolism* Protein Binding / drug effects RNA-Binding Proteins / metabolism* Sulfhydryl Compounds / metabolism*
IF 1.516
引用数 0
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY
リソース情報
ヒト・動物細胞