RRC ID |
4419
|
著者 |
Nakamura S, Saito D, Tanaka M.
|
タイトル |
Generation of transgenic medaka using modified bacterial artificial chromosome.
|
ジャーナル |
Dev Growth Differ
|
Abstract |
The availability of bacterial artificial chromosome (BAC) offers a good genomic platform for a targeted integration of an exogenous gene by a homologous recombination system in Escherichia coli. In combination with microinjection technology, this system allows for the analysis of various aspects of biological phenomena occurring in vivo using Japanese medaka fish (Oryzias latipes). Here we describe a streamlined procedure for selecting BAC clones based on the medaka University of Tokyo genome browser (UTGB), followed by rapid modification with enhanced green fluorescent protein (EGFP) or DsRed fragments for transgenic analysis in medaka. Experimental procedures for BAC DNA preparation, microinjection of medaka embryos and screening of resulting transgenic medaka carrying EGFP/DsRed modified BAC clones are also described.
|
巻・号 |
50(6)
|
ページ |
415-9
|
公開日 |
2008-8-1
|
DOI |
10.1111/j.1440-169X.2008.01027.x
|
PII |
DGD1027
|
PMID |
18422685
|
MeSH |
Animals
Animals, Genetically Modified*
Chromosomes, Artificial, Bacterial*
Developmental Biology / methods
Escherichia coli
Genetic Techniques
Genome
Green Fluorescent Proteins / metabolism
Models, Genetic
Oryzias / genetics*
Recombination, Genetic
Transgenes
|
IF |
1.723
|
引用数 |
35
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
リソース情報 |
メダカ |
BAC clones (golwb014_G01) |