論文 - 詳細
| RRC ID | 44257 |
|---|---|
| 著者 | Kloesch B, Becker T, Dietersdorfer E, Kiener H, Steiner G. |
| タイトル | Anti-inflammatory and apoptotic effects of the polyphenol curcumin on human fibroblast-like synoviocytes. |
| ジャーナル | Int Immunopharmacol |
| Abstract |
BACKGROUND:It has recently been reported that the polyphenol curcumin has pronounced anti-carcinogenic, anti-inflammatory and pro-apoptotic properties. This study investigated possible anti-inflammatory and apoptotic effects of curcumin on the human synovial fibroblast cell line MH7A, and on fibroblast-like synoviocytes (FLS) derived from patients with rheumatoid arthritis (RA). METHODS:MH7A cells and RA-FLS were stimulated either with interleukin (IL)-1β or phorbol 12-myristate 13 acetate (PMA), and treated simultaneously or sequentially with increasing concentrations of curcumin. Release of interleukin (IL)-6 and vascular endothelial growth factor (VEGF)-A was quantified by enzyme-linked immunosorbent assays (ELISAs). In MH7A cells, modulation of the transcription factor nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinases (MAPKs) such as p38 and extracellular-signal regulated kinase (ERK1/2) were analysed by a reporter gene assay and Western blot, respectively. Pro-apoptotic events were monitored by Annexin-V/7-AAD based assay. Cleavage of pro-caspase-3 and -7 was checked with specific antibodies. RESULTS:Curcumin effectively blocked IL-1β and PMA-induced IL-6 expression both in MH7A cells and RA-FLS. VEGF-A expression could only be detected in RA-FLS and was induced by PMA, but not by IL-1β. Furthermore, curcumin inhibited activation of NF-κB and induced dephosphorylation of ERK1/2. Treatment of FLS with high concentrations of curcumin was associated with a decrease in cell viability and induction of apoptosis. CONCLUSION:The natural compound curcumin represents strong anti-inflammatory properties and induces apoptosis in FLS. This study provides an insight into possible molecular mechanisms of this substance and suggests it as a natural remedy for the treatment of chronic inflammatory diseases like RA. |
| 巻・号 | 15(2) |
| ページ | 400-5 |
| 公開日 | 2013-2-1 |
| DOI | 10.1016/j.intimp.2013.01.003 |
| PII | S1567-5769(13)00009-X |
| PMID | 23347846 |
| MeSH | Anti-Inflammatory Agents, Non-Steroidal / administration & dosage* Anti-Inflammatory Agents, Non-Steroidal / adverse effects Apoptosis / drug effects Arthritis, Rheumatoid / drug therapy* Arthritis, Rheumatoid / immunology Cell Line Curcumin / administration & dosage* Curcumin / adverse effects Extracellular Signal-Regulated MAP Kinases / metabolism Fibroblasts / drug effects* Fibroblasts / pathology Humans Interleukin-1beta / immunology Interleukin-6 / metabolism NF-kappa B / metabolism Polyphenols / administration & dosage* Polyphenols / adverse effects Synovial Membrane / pathology Tetradecanoylphorbol Acetate / analogs & derivatives Tetradecanoylphorbol Acetate / immunology Vascular Endothelial Growth Factor A / metabolism |
| IF | 3.943 |
| 引用数 | 64 |
| WOS 分野 | PHARMACOLOGY & PHARMACY IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |