論文 - 詳細
| RRC ID | 44287 |
|---|---|
| 著者 | Katsuda T, Tsuchiya R, Kosaka N, Yoshioka Y, Takagaki K, Oki K, Takeshita F, Sakai Y, Kuroda M, Ochiya T. |
| タイトル | Human adipose tissue-derived mesenchymal stem cells secrete functional neprilysin-bound exosomes. |
| ジャーナル | Sci Rep |
| Abstract |
Alzheimer's disease (AD) is characterized by the accumulation of β-amyloid peptide (Aβ) in the brain because of an imbalance between Aβ production and clearance. Neprilysin (NEP) is the most important Aβ-degrading enzyme in the brain. Thus, researchers have explored virus-mediated NEP gene delivery. However, such strategies may entail unexpected risks, and thus exploration of a new possibility for NEP delivery is also required. Here, we show that human adipose tissue-derived mesenchymal stem cells (ADSCs) secrete exosomes carrying enzymatically active NEP. The NEP-specific activity level of 1 μg protein from ADSC-derived exosomes was equivalent to that of ~ 0.3 ng of recombinant human NEP. Of note, ADSC-derived exosomes were transferred into N2a cells, and were suggested to decrease both secreted and intracellular Aβ levels in the N2a cells. Importantly, these characteristics were more pronounced in ADSCs than bone marrow-derived mesenchymal stem cells, suggesting the therapeutic relevance of ADSC-derived exosomes for AD. |
| 巻・号 | 3 |
| ページ | 1197 |
| 公開日 | 2013-1-1 |
| DOI | 10.1038/srep01197 |
| PII | srep01197 |
| PMID | 23378928 |
| PMC | PMC3561625 |
| MeSH | Adipose Tissue / cytology* Alzheimer Disease / metabolism Alzheimer Disease / pathology Amyloid beta-Peptides / metabolism Cells, Cultured Exosomes / metabolism* Fluorescent Dyes / chemistry Humans Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / metabolism* Neprilysin / genetics Neprilysin / metabolism* Peptide Fragments / metabolism Recombinant Proteins / biosynthesis Recombinant Proteins / genetics |
| IF | 3.998 |
| 引用数 | 200 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 32 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | BM-MSCs |