RRC ID 44290
Author Liu H, Han D, Liu Y, Hou X, Wu J, Li H, Yang J, Shen C, Yang G, Fu C, Li X, Che H, Ai J, Zhao S.
Title Harmine hydrochloride inhibits Akt phosphorylation and depletes the pool of cancer stem-like cells of glioblastoma.
Journal J. Neurooncol.
Abstract Harmine hydrochloride (Har-hc), a derivative from Harmine which is a natural extractive from plants, has been considered for treatment of kinds of cancers and cerebral diseases. In this study, we found that Har-hc clearly decreased cell viability, induced apoptosis and inhibited Akt phosphorylation in glioblastoma cell lines. Moreover, Har-hc had the ability to inhibit self-renewal and promote differentiation of glioblastoma stem like cells (GSLCs) accompanied by inhibition of Akt phosphorylation. Especially, we demonstrated that Har-hc inhibited neurosphere formation of human primary GSLCs. In vivo test also confirmed Har-hc decreased the tumorigenicity of GSLCs. Thus we conclude that Har-hc has potent anti-cancer effects in glioblastoma cells, which is at least partially via inhibition of Akt phosphorylation. Administration of Har-hc may act as a new approach to glioblastoma treatment.
Volume 112(1)
Pages 39-48
Published 2013-3
DOI 10.1007/s11060-012-1034-x
PMID 23392846
MeSH Analysis of Variance Animals Apoptosis / drug effects Brain / drug effects Brain / pathology Cell Differentiation / drug effects Cell Line, Tumor Cell Proliferation / drug effects* Chromones / pharmacology Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Gene Expression Regulation, Neoplastic / drug effects Glioblastoma / pathology* Harmine / pharmacology* Humans Monoamine Oxidase Inhibitors / pharmacology* Morpholines / pharmacology Neoplastic Stem Cells / drug effects* Neoplastic Stem Cells / pathology Nerve Tissue Proteins / metabolism Oncogene Protein v-akt / genetics Oncogene Protein v-akt / metabolism Phosphorylation / drug effects Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / metabolism Signal Transduction Time Factors bcl-2-Associated X Protein / genetics bcl-2-Associated X Protein / metabolism
IF 3.06
Times Cited 9
WOS Category CLINICAL NEUROLOGY ONCOLOGY
Resource
Human and Animal Cells