Reference - Detail
|Author||Liu H, Han D, Liu Y, Hou X, Wu J, Li H, Yang J, Shen C, Yang G, Fu C, Li X, Che H, Ai J, Zhao S.|
|Title||Harmine hydrochloride inhibits Akt phosphorylation and depletes the pool of cancer stem-like cells of glioblastoma.|
Harmine hydrochloride (Har-hc), a derivative from Harmine which is a natural extractive from plants, has been considered for treatment of kinds of cancers and cerebral diseases. In this study, we found that Har-hc clearly decreased cell viability, induced apoptosis and inhibited Akt phosphorylation in glioblastoma cell lines. Moreover, Har-hc had the ability to inhibit self-renewal and promote differentiation of glioblastoma stem like cells (GSLCs) accompanied by inhibition of Akt phosphorylation. Especially, we demonstrated that Har-hc inhibited neurosphere formation of human primary GSLCs. In vivo test also confirmed Har-hc decreased the tumorigenicity of GSLCs. Thus we conclude that Har-hc has potent anti-cancer effects in glioblastoma cells, which is at least partially via inhibition of Akt phosphorylation. Administration of Har-hc may act as a new approach to glioblastoma treatment.
|MeSH||Analysis of Variance Animals Apoptosis / drug effects Brain / drug effects Brain / pathology Cell Differentiation / drug effects Cell Line, Tumor Cell Proliferation / drug effects* Chromones / pharmacology Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Gene Expression Regulation, Neoplastic / drug effects Glioblastoma / pathology* Harmine / pharmacology* Humans Monoamine Oxidase Inhibitors / pharmacology* Morpholines / pharmacology Neoplastic Stem Cells / drug effects* Neoplastic Stem Cells / pathology Nerve Tissue Proteins / metabolism Oncogene Protein v-akt / genetics Oncogene Protein v-akt / metabolism Phosphorylation / drug effects Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / metabolism Signal Transduction Time Factors bcl-2-Associated X Protein / genetics bcl-2-Associated X Protein / metabolism|
|WOS Category||CLINICAL NEUROLOGY ONCOLOGY|
|Human and Animal Cells|