| RRC ID |
44502
|
| Author |
Usui T, Okada M, Hara Y, Yamawaki H.
|
| Title |
Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation.
|
| Journal |
Am J Physiol Heart Circ Physiol
|
| Abstract |
Eukaryotic elongation factor 2 kinase (eEF2K) is a Ca2+/calmodulin-dependent protein kinase. We recently demonstrated that eEF2K protein increases in mesenteric artery from spontaneously hypertensive rats (SHR). Pathogenesis of hypertension is regulated in part by vascular inflammation. We tested the hypothesis whether eEF2K mediates vascular inflammatory responses and development of hypertension. In vascular endothelial cells, small interfering RNA (siRNA) against eEF2K inhibited induction of VCAM-1 and endothelial-selectin as well as monocyte adhesion by TNF-α (10 ng/ml). eEF2K siRNA inhibited phosphorylation of JNK and NF-κB p65 as well as reactive oxygen species (ROS) production by TNF-α. In vascular smooth muscle cells, eEF2K siRNA also inhibited VCAM-1 induction and phosphorylation of JNK and NF-κB by TNF-α. In vivo, increased blood pressure in SHR and ROS production, induction of inflammatory molecules, and hypertrophy in SHR superior mesenteric artery were reduced by an eEF2K inhibitor NH125 (500 μg·kg(-1)·day(-1)). In SHR superior mesenteric artery, impairment of ACh-induced relaxation was normalized by NH125. The present results for the first time demonstrate that eEF2K mediates TNF-α-induced vascular inflammation via ROS-dependent mechanism, which is at least partly responsible for the development of hypertension in SHR.
|
| Volume |
305(5)
|
| Pages |
H756-68
|
| Published |
2013-9-1
|
| DOI |
10.1152/ajpheart.00373.2013
|
| PII |
ajpheart.00373.2013
|
| PMID |
23812389
|
| PMC |
PMC4073962
|
| MeSH |
Animals
Blood Pressure / drug effects
Blood Pressure / physiology
Cells, Cultured
Disease Models, Animal
Elongation Factor 2 Kinase / antagonists & inhibitors
Elongation Factor 2 Kinase / drug effects
Elongation Factor 2 Kinase / physiology*
Endothelium, Vascular / drug effects
Endothelium, Vascular / metabolism
Endothelium, Vascular / pathology
Humans
Hypertension / metabolism
Hypertension / physiopathology*
Imidazoles / pharmacology
MAP Kinase Kinase 4 / metabolism
Male
NF-kappa B / metabolism
Oxidative Stress / physiology*
RNA, Small Interfering / pharmacology
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Rats, Wistar
Reactive Oxygen Species / metabolism
Tumor Necrosis Factor-alpha / adverse effects*
Tumor Necrosis Factor-alpha / pharmacology
Vascular Cell Adhesion Molecule-1 / metabolism
Vasculitis / chemically induced*
Vasculitis / metabolism
Vasculitis / physiopathology*
|
| IF |
3.864
|
| Times Cited |
24
|
|
WOS Category
|
PHYSIOLOGY
CARDIAC & CARDIOVASCULAR SYSTEMS
PERIPHERAL VASCULAR DISEASE
|
| Resource |
| Human and Animal Cells |
U937 |
