論文 - 詳細
| RRC ID | 44515 |
|---|---|
| 著者 | Patel V, Chen LS, Wierda WG, Balakrishnan K, Gandhi V. |
| タイトル | Impact of bone marrow stromal cells on Bcl-2 family members in chronic lymphocytic leukemia. |
| ジャーナル | Leuk Lymphoma |
| Abstract |
Chronic lymphocytic leukemia (CLL) is the most common form of adult leukemia in the Western world. High levels of Bcl-2 family anti-apoptotic proteins are responsible for apoptosis resistance. Besides anti-apoptotic proteins, the microenvironment provides substantial survival signals to CLL leukemic cells. However, in-depth knowledge on the role of individual Bcl-2 family members in the context of the microenvironment is still limited. We performed a comprehensive analysis of transcripts and proteins of 18 Bcl-2 family members using an "apoptosis array microfluidic card" in primary cells before and after stromal co-cultures. Our data showed that five of six anti-apoptotic members (excluding Bcl-b), two of three pro-apoptotic members (excluding Bok) and six of nine BH3-only members were present at detectable mRNA levels in CLL cells. Importantly, stromal-mediated extended survival of CLL cells was strongly associated with elevated global transcription. Upon co-culturing with stromal cells, there was an early response of an increase in anti- (2/5) and pro-apoptotic protein (3/8) transcripts on day 1, while an increase in anti-apoptotic proteins was observed on day 3, with no significant change in pro-apoptotic proteins. Our study revealed a differential pattern of expression of both transcripts and proteins following stromal co-cultures, proposing a significance of Bcl-2 family members in the stromal microenvironment. |
| 巻・号 | 55(4) |
| ページ | 899-910 |
| 公開日 | 2014-4-1 |
| DOI | 10.3109/10428194.2013.819573 |
| PMID | 23837491 |
| PMC | PMC4172382 |
| MeSH | Apoptosis / genetics Cell Survival Coculture Techniques Gene Expression Profiling Humans Leukemia, Lymphocytic, Chronic, B-Cell / genetics* Leukemia, Lymphocytic, Chronic, B-Cell / metabolism Mesenchymal Stem Cells / metabolism* Proto-Oncogene Proteins c-bcl-2 / genetics* Proto-Oncogene Proteins c-bcl-2 / metabolism RNA, Messenger / genetics Transcription, Genetic |
| IF | 2.969 |
| 引用数 | 14 |
| WOS 分野 | ONCOLOGY HEMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | StromaNKtert(RCB2350) |