| RRC ID |
44544
|
| Author |
Itoh T, Tsuzuki R, Tanaka T, Ninomiya M, Yamaguchi Y, Takenaka H, Ando M, Tsukamasa Y, Koketsu M.
|
| Title |
Reduced scytonemin isolated from Nostoc commune induces autophagic cell death in human T-lymphoid cell line Jurkat cells.
|
| Journal |
Food Chem Toxicol
|
| Abstract |
Nostoc commune is a terrestrial benthic blue-green alga that often forms an extended mucilaginous layer on the soil, accumulates on stones and mud in aquatic environments. Reduced-scytonemin (R-scy), isolated from N. commune Vaucher, has been shown to suppress the human T-lymphoid Jurkat cell growth. To reveal the mechanisms underlying the R-scy-mediated inhibition of Jurkat cell growth, we examined cell morphology, DNA fragmentation, and microtubule-associated protein light chain 3 (LC3) modification in these cells. We observed multiple vacuoles as well as the conversion of LC3-I to LC3-II in R-scy-treated cells. These results suggest that the R-scy induced Jurkat cell growth inhibition is attributable to the induction of type II programmed cell death (PCD II; autophagic cell death or autophagy). We further examined the mechanisms underlying R-scy-induced PCDII. The cells treated with R-scy produced large amounts of reactive oxygen species (ROS), leading to the induction of mitochondrial dysfunction. However, the elimination of R-scy-induced ROS by treatment with N-acetyl-L-cysteine (NAC) markedly opposed R-scy-induced PCDII. Based on these results, we conclude that ROS formation plays a critical role in R-scy-induced PCDII.
|
| Volume |
60
|
| Pages |
76-82
|
| Published |
2013-10-1
|
| DOI |
10.1016/j.fct.2013.07.016
|
| PII |
S0278-6915(13)00471-7
|
| PMID |
23876822
|
| MeSH |
Autophagy / drug effects*
Cell Line
Humans
Indoles / chemistry*
Indoles / isolation & purification*
Jurkat Cells
Microtubule-Associated Proteins / metabolism
Mitochondria / drug effects
Mitochondria / metabolism
Nostoc commune / chemistry*
Oxidative Stress / drug effects
Phenols / chemistry*
Phenols / isolation & purification*
Reactive Oxygen Species / metabolism
Vacuoles / metabolism
|
| IF |
4.679
|
| Times Cited |
14
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
TOXICOLOGY
|
| Resource |
| Human and Animal Cells |
Jurkat |
