論文 - 詳細
| RRC ID | 44544 |
|---|---|
| 著者 | Itoh T, Tsuzuki R, Tanaka T, Ninomiya M, Yamaguchi Y, Takenaka H, Ando M, Tsukamasa Y, Koketsu M. |
| タイトル | Reduced scytonemin isolated from Nostoc commune induces autophagic cell death in human T-lymphoid cell line Jurkat cells. |
| ジャーナル | Food Chem Toxicol |
| Abstract |
Nostoc commune is a terrestrial benthic blue-green alga that often forms an extended mucilaginous layer on the soil, accumulates on stones and mud in aquatic environments. Reduced-scytonemin (R-scy), isolated from N. commune Vaucher, has been shown to suppress the human T-lymphoid Jurkat cell growth. To reveal the mechanisms underlying the R-scy-mediated inhibition of Jurkat cell growth, we examined cell morphology, DNA fragmentation, and microtubule-associated protein light chain 3 (LC3) modification in these cells. We observed multiple vacuoles as well as the conversion of LC3-I to LC3-II in R-scy-treated cells. These results suggest that the R-scy induced Jurkat cell growth inhibition is attributable to the induction of type II programmed cell death (PCD II; autophagic cell death or autophagy). We further examined the mechanisms underlying R-scy-induced PCDII. The cells treated with R-scy produced large amounts of reactive oxygen species (ROS), leading to the induction of mitochondrial dysfunction. However, the elimination of R-scy-induced ROS by treatment with N-acetyl-L-cysteine (NAC) markedly opposed R-scy-induced PCDII. Based on these results, we conclude that ROS formation plays a critical role in R-scy-induced PCDII. |
| 巻・号 | 60 |
| ページ | 76-82 |
| 公開日 | 2013-10-1 |
| DOI | 10.1016/j.fct.2013.07.016 |
| PII | S0278-6915(13)00471-7 |
| PMID | 23876822 |
| MeSH | Autophagy / drug effects* Cell Line Humans Indoles / chemistry* Indoles / isolation & purification* Jurkat Cells Microtubule-Associated Proteins / metabolism Mitochondria / drug effects Mitochondria / metabolism Nostoc commune / chemistry* Oxidative Stress / drug effects Phenols / chemistry* Phenols / isolation & purification* Reactive Oxygen Species / metabolism Vacuoles / metabolism |
| IF | 4.679 |
| 引用数 | 14 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY TOXICOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat |