論文 - 詳細
| RRC ID | 4466 |
|---|---|
| 著者 | Rocheleau CE, Cullison K, Huang K, Bernstein Y, Spilker AC, Sundaram MV. |
| タイトル | The Caenorhabditis elegans ekl (enhancer of ksr-1 lethality) genes include putative components of a germline small RNA pathway. |
| ジャーナル | Genetics |
| Abstract |
A canonical Ras-ERK signaling pathway specifies the fate of the excretory duct cell during Caenorhabditis elegans embryogenesis. The paralogs ksr-1 and ksr-2 encode scaffolding proteins that facilitate signaling through this pathway and that act redundantly to promote the excretory duct fate. In a genomewide RNAi screen for genes that, like ksr-2, are required in combination with ksr-1 for the excretory duct cell fate, we identified 16 "ekl" (enhancer of ksr-1 lethality) genes that are largely maternally required and that have molecular identities suggesting roles in transcriptional or post-transcriptional gene regulation. These include the Argonaute gene csr-1 and a specific subset of other genes implicated in endogenous small RNA processes, orthologs of multiple components of the NuA4/Tip60 histone acetyltransferase and CCR4/NOT deadenylase complexes, and conserved enzymes involved in ubiquitination and deubiquitination. The identification of four small RNA regulators (csr-1, drh-3, ego-1, and ekl-1) that share the Ekl phenotype suggests that these genes define a functional pathway required for the production and/or function of particular germline small RNA(s). These small RNAs and the other ekl genes likely control the expression of one or more regulators of Ras-ERK signaling that function at or near the level of kinase suppressor of Ras (KSR). |
| 巻・号 | 178(3) |
| ページ | 1431-43 |
| 公開日 | 2008-3-1 |
| DOI | 10.1534/genetics.107.084608 |
| PII | genetics.107.084608 |
| PMID | 18245826 |
| PMC | PMC2278074 |
| MeSH | Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Conserved Sequence Epistasis, Genetic Gene Deletion Genes, Helminth* Germ Cells / metabolism* Histone Acetyltransferases / metabolism Larva Models, Genetic Mutation / genetics Protein Kinases / metabolism RNA Interference RNA, Helminth / genetics* RNA-Induced Silencing Complex Ubiquitination ras Proteins / metabolism |
| IF | 4.015 |
| 引用数 | 34 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1531 tm1312 tm2531 |