論文 - 詳細
RRC ID | 4469 |
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著者 | Bounoutas A, Zheng Q, Nonet ML, Chalfie M. |
タイトル | mec-15 encodes an F-box protein required for touch receptor neuron mechanosensation, synapse formation and development. |
ジャーナル | Genetics |
Abstract |
Selective protein degradation is a key regulator of neuronal development and synaptogenesis. Complexes that target proteins for degradation often contain F-box proteins. Here we characterize MEC-15, an F-box protein with WD repeats, which is required for the development and function of Caenorhabditis elegans touch receptor neurons (TRNs). Mutations in mec-15 produce defects in TRN touch sensitivity, chemical synapse formation, and cell-body morphology. All mec-15 mutant phenotypes are enhanced by mutations in a MAP kinase pathway composed of the MAPKKK DLK-1, the MAPKK MKK-4, and the p38 MAPK PMK-3. A mutation of the rpm-1 gene, which encodes an E3 ubiquitin ligase that negatively regulates this pathway to promote synaptogenesis, suppresses only the mec-15 cell-body defect. Thus, MEC-15 acts in parallel with RPM-1, implicating a second protein degradation pathway in TRN development. In addition, all mec-15 phenotypes can be dominantly suppressed by mutations in mec-7, which encodes a beta-tubulin, and dominantly enhanced by mutations in mec-12, which encodes an alpha-tubulin. Since mec-15 phenotypes depend on the relative levels of these tubulins, MEC-15 may target proteins whose function is affected by these levels. |
巻・号 | 183(2) |
ページ | 607-17, 1SI-4SI |
公開日 | 2009-10-1 |
DOI | 10.1534/genetics.109.105726 |
PII | genetics.109.105726 |
PMID | 19652181 |
PMC | PMC2766320 |
MeSH | Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* F-Box Proteins / genetics F-Box Proteins / metabolism F-Box Proteins / physiology* Gene Expression Regulation Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Mechanoreceptors / cytology Mechanoreceptors / metabolism Mechanoreceptors / physiology* Mechanotransduction, Cellular / genetics Mechanotransduction, Cellular / physiology Microscopy, Fluorescence Mutation Neurons / cytology Neurons / metabolism Neurons / physiology* Phenotype Synapses / physiology Touch / physiology* Tubulin / genetics Tubulin / metabolism p38 Mitogen-Activated Protein Kinases / genetics p38 Mitogen-Activated Protein Kinases / metabolism |
IF | 4.015 |
引用数 | 24 |
WOS 分野 | GENETICS & HEREDITY |
リソース情報 | |
線虫 | tm2691 |