RRC ID |
44889
|
著者 |
Angcajas AB, Hirai N, Kaneshiro K, Karim MR, Horii Y, Kubota M, Fujimura S, Kadowaki M.
|
タイトル |
Diversity of amino acid signaling pathways on autophagy regulation: a novel pathway for arginine.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Autophagy is the intracellular bulk degradation process to eliminate damaged cellular machinery and to recycle building blocks, and is crucial for cell survival and cell death. Amino acids modulate autophagy in response to nutrient starvation and oxidative stress. We investigated the relevance of reactive oxygen species (ROS) production on the regulation of autophagy using amino acids, both as a mixture and individually, in rat hepatoma H4-II-E cells. Nutrient starvation elevated ROS production and stimulated autophagy. Treatment with complete (CAA), regulatory (RegAA) and non-regulatory (NonRegAA) amino acid mixtures showed significant suppression of ROS production, whereas only CAA and RegAA exhibited significant suppression of autophagy, suggesting a dissociation of the two responses. The effects of individual amino acids were examined. Leucine from RegAA decreased ROS production and suppressed autophagy. However, methionine and proline from RegAA and arginine, cystine and glutamic acid from NonRegAA suppressed autophagy with an opposite increase in ROS production. Other amino acids from the NonRegAA group showed stimulating effects on ROS production without an autophagic response. Arginine's effect on autophagy suppression was not blocked by rapamycin, indicating an mTOR-independent pathway. Inhibitor studies on arginine-regulated autophagy may indicate the involvement of NO pathway, which is independent from ROS and mTOR pathways.
|
巻・号 |
446(1)
|
ページ |
8-14
|
公開日 |
2014-3-28
|
DOI |
10.1016/j.bbrc.2014.01.117
|
PII |
S0006-291X(14)00152-1
|
PMID |
24486546
|
MeSH |
Amino Acids / metabolism*
Animals
Arginine / metabolism*
Autophagy / drug effects
Autophagy / physiology*
Cell Line
Hep G2 Cells
Humans
Nitric Oxide / metabolism
Rats
Reactive Oxygen Species / metabolism
Signal Transduction
Sirolimus / pharmacology
TOR Serine-Threonine Kinases / antagonists & inhibitors
TOR Serine-Threonine Kinases / metabolism
|
IF |
2.985
|
引用数 |
12
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
Hep G2 |