RRC ID 44937
著者 Nagahama M, Takahashi C, Aoyanagi K, Tashiro R, Kobayashi K, Sakaguchi Y, Ishidoh K, Sakurai J.
タイトル Intracellular trafficking of Clostridium botulinum C2 toxin.
ジャーナル Toxicon
Abstract Clostridium botulinum C2 toxin is a binary toxin composed of an enzymatic component (C2I) and binding component (C2II). The activated binding component (C2IIa) forms heptamers and the oligomer with C2I is taken up by receptor-mediated endocytosis. We investigated the intracellular trafficking of C2 toxin. When MDCK cells were incubated with C2I and C2IIa at 37 °C, C2I colocalized with C2IIa in cytoplasmic vesicles at 5 min, and C2I then disappeared (15 min incubation and later), and C2IIa was observed in the vesicles. Internalized C2I and C2IIa were transported to early endosomes. Some of both components were returned to the plasma membrane through recycling endosomes, whereas the rest of C2IIa was transported to late endosomes and lysosomes for degradation. Bafilomycin A1, an endosomal acidification inhibitor, caused the accumulation of C2IIa in endosomes, and both nocodazole and colchicine, microtubule-disrupting agents, restricted C2IIa's movement in the cytosol. These results indicated that an internalized C2I and C2IIa complex was delivered to early endosomes, and that subsequent delivery of C2I to the cytoplasm occurred in early endosomes. C2IIa was either sent back to the plasma membranes through recycling endosomes or transported to late endosomes and lysosomes for degradation.
巻・号 82
ページ 76-82
公開日 2014-5-1
DOI 10.1016/j.toxicon.2014.02.009
PII S0041-0101(14)00053-1
PMID 24582943
MeSH Animals Botulinum Toxins / antagonists & inhibitors Botulinum Toxins / metabolism* Botulinum Toxins / toxicity Cell Line Cell Membrane / metabolism Cell Survival / drug effects Cytoplasm / metabolism Dogs Endocytosis / drug effects Endosomes / metabolism Macrolides / pharmacology Recombinant Proteins / metabolism
IF 2.201
引用数 8
WOS 分野 PHARMACOLOGY & PHARMACY TOXICOLOGY
リソース情報
ヒト・動物細胞 MDCK(RCB0995)