RRC ID 44964
Author Tsujiura M, Komatsu S, Ichikawa D, Shiozaki A, Konishi H, Takeshita H, Moriumura R, Nagata H, Kawaguchi T, Hirajima S, Arita T, Fujiwara H, Okamoto K, Otsuji E.
Title Circulating miR-18a in plasma contributes to cancer detection and monitoring in patients with gastric cancer.
Journal Gastric Cancer
Abstract BACKGROUND:Recently, circulating microRNAs have been reported to be stably detectable in plasma/serum and to function as potent non-invasive biomarkers in various cancers. We hypothesized that miR-18a could contribute to a novel plasma biomarker in patients with gastric cancer (GC).
METHODS:We focused on miR-18a, which is a component of miR-17-92 cluster and has been reported as highly expressed in GC tissues. The study involved three steps: (1) confirmation of the higher miR-18a expression in primary GC tissues and GC cell lines than in normal gastric tissues and a fibroblast cell line; (2) evaluation of the plasma miR-18a assay using quantitative RT-PCR by comparing 104 GC patients and 65 healthy volunteers; (3) evaluation of monitoring tumor dynamics by the plasma miR-18a assay.
RESULTS:(1) The miR-18a expressions were significantly higher in GC tissues than in normal gastric tissues (P = 0.0286) and higher in all examined GC cell lines than in the fibroblast cell line. (2) The plasma miR-18a concentrations were significantly higher in GC patients than in healthy controls (P < 0.0001). The value of the area under the receiver-operating characteristic curve was 0.8059. (3) The plasma miR-18a levels were significantly reduced in postoperative samples compared to in preoperative samples (P = 0.0002). In an miR-18a overexpressing cell line, the miR-18a concentration of cultured medium increased in both cell number and time-course dependent manners, suggesting microRNA might be released from cancer cells into the surrounding environment.
CONCLUSIONS:Circulating miR-18a could be a useful biomarker for screening GC and monitoring tumor dynamics.
Volume 18(2)
Pages 271-9
Published 2015-4-1
DOI 10.1007/s10120-014-0363-1
PMID 24626859
MeSH Adenocarcinoma / blood Adenocarcinoma / genetics* Adenocarcinoma / pathology Adult Aged Aged, 80 and over Biomarkers, Tumor / blood Biomarkers, Tumor / genetics* Case-Control Studies Cell Differentiation Cell Proliferation Cells, Cultured Early Detection of Cancer Female Fibroblasts Follow-Up Studies Humans Male MicroRNAs / blood MicroRNAs / genetics* Middle Aged Neoplasm Invasiveness Neoplasm Recurrence, Local / blood Neoplasm Recurrence, Local / genetics* Neoplasm Recurrence, Local / pathology Neoplasm Staging Prognosis ROC Curve Real-Time Polymerase Chain Reaction Stomach Neoplasms / blood Stomach Neoplasms / genetics* Stomach Neoplasms / pathology
IF 5.554
Times Cited 59
Human and Animal Cells