Reference - Detail
RRC ID | 44964 |
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Author | Tsujiura M, Komatsu S, Ichikawa D, Shiozaki A, Konishi H, Takeshita H, Moriumura R, Nagata H, Kawaguchi T, Hirajima S, Arita T, Fujiwara H, Okamoto K, Otsuji E. |
Title | Circulating miR-18a in plasma contributes to cancer detection and monitoring in patients with gastric cancer. |
Journal | Gastric Cancer |
Abstract |
BACKGROUND:Recently, circulating microRNAs have been reported to be stably detectable in plasma/serum and to function as potent non-invasive biomarkers in various cancers. We hypothesized that miR-18a could contribute to a novel plasma biomarker in patients with gastric cancer (GC). METHODS:We focused on miR-18a, which is a component of miR-17-92 cluster and has been reported as highly expressed in GC tissues. The study involved three steps: (1) confirmation of the higher miR-18a expression in primary GC tissues and GC cell lines than in normal gastric tissues and a fibroblast cell line; (2) evaluation of the plasma miR-18a assay using quantitative RT-PCR by comparing 104 GC patients and 65 healthy volunteers; (3) evaluation of monitoring tumor dynamics by the plasma miR-18a assay. RESULTS:(1) The miR-18a expressions were significantly higher in GC tissues than in normal gastric tissues (P = 0.0286) and higher in all examined GC cell lines than in the fibroblast cell line. (2) The plasma miR-18a concentrations were significantly higher in GC patients than in healthy controls (P < 0.0001). The value of the area under the receiver-operating characteristic curve was 0.8059. (3) The plasma miR-18a levels were significantly reduced in postoperative samples compared to in preoperative samples (P = 0.0002). In an miR-18a overexpressing cell line, the miR-18a concentration of cultured medium increased in both cell number and time-course dependent manners, suggesting microRNA might be released from cancer cells into the surrounding environment. CONCLUSIONS:Circulating miR-18a could be a useful biomarker for screening GC and monitoring tumor dynamics. |
Volume | 18(2) |
Pages | 271-9 |
Published | 2015-4-1 |
DOI | 10.1007/s10120-014-0363-1 |
PMID | 24626859 |
MeSH | Adenocarcinoma / blood Adenocarcinoma / genetics* Adenocarcinoma / pathology Adult Aged Aged, 80 and over Biomarkers, Tumor / blood Biomarkers, Tumor / genetics* Case-Control Studies Cell Differentiation Cell Proliferation Cells, Cultured Early Detection of Cancer Female Fibroblasts Follow-Up Studies Humans Male MicroRNAs / blood MicroRNAs / genetics* Middle Aged Neoplasm Invasiveness Neoplasm Recurrence, Local / blood Neoplasm Recurrence, Local / genetics* Neoplasm Recurrence, Local / pathology Neoplasm Staging Prognosis ROC Curve Real-Time Polymerase Chain Reaction Stomach Neoplasms / blood Stomach Neoplasms / genetics* Stomach Neoplasms / pathology |
IF | 7.088 |
Times Cited | 59 |
WOS Category | ONCOLOGY GASTROENTEROLOGY & HEPATOLOGY |
Resource | |
Human and Animal Cells | WI-38(RCB0702) |