Reference - Detail
|Author||Kogiso T, Nagahara H, Hashimoto E, Ariizumi S, Yamamoto M, Shiratori K.|
|Title||Efficient induction of apoptosis by wee1 kinase inhibition in hepatocellular carcinoma cells.|
Transforming growth factor-β1 (TGF-β1) potently inhibits human hepatocellular carcinoma (HCC) cell growth. Here we demonstrated that TGF-β1-induced apoptosis is mediated by decreased phosphorylation of cdc2 at Tyr15 accompanied by down-regulation of Wee1 kinase expression. As expected from these results, a Wee1 kinase inhibitor efficiently induced apoptosis in HCC cells in the absence of TGF-β1 treatment. In surgically resected samples, Wee1 kinase was expressed in moderately to poorly differentiated HCC, whereas no Wee1 kinase expression was observed in non-cancerous tissue, including cirrhotic tissue. Our results suggest that Wee1 kinase inhibitors may be a practical novel therapeutic option against advanced HCC.
|MeSH||Apoptosis / drug effects* CDC2 Protein Kinase Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / metabolism* Cell Cycle Proteins / antagonists & inhibitors* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Line, Tumor Cyclin-Dependent Kinases / metabolism Enzyme Activation / drug effects Gene Expression Humans Immunohistochemistry Liver Neoplasms / genetics Liver Neoplasms / metabolism* Nuclear Proteins / antagonists & inhibitors* Nuclear Proteins / genetics Nuclear Proteins / metabolism Protein Kinase Inhibitors / pharmacology* Protein-Tyrosine Kinases / antagonists & inhibitors* Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism RNA Interference Transforming Growth Factor beta1 / pharmacology|
|WOS Category||CELL BIOLOGY|
|Human and Animal Cells|