論文 - 詳細
| RRC ID | 45137 |
|---|---|
| 著者 | Kogiso T, Nagahara H, Hashimoto E, Ariizumi S, Yamamoto M, Shiratori K. |
| タイトル | Efficient induction of apoptosis by wee1 kinase inhibition in hepatocellular carcinoma cells. |
| ジャーナル | PLoS One |
| Abstract |
Transforming growth factor-β1 (TGF-β1) potently inhibits human hepatocellular carcinoma (HCC) cell growth. Here we demonstrated that TGF-β1-induced apoptosis is mediated by decreased phosphorylation of cdc2 at Tyr15 accompanied by down-regulation of Wee1 kinase expression. As expected from these results, a Wee1 kinase inhibitor efficiently induced apoptosis in HCC cells in the absence of TGF-β1 treatment. In surgically resected samples, Wee1 kinase was expressed in moderately to poorly differentiated HCC, whereas no Wee1 kinase expression was observed in non-cancerous tissue, including cirrhotic tissue. Our results suggest that Wee1 kinase inhibitors may be a practical novel therapeutic option against advanced HCC. |
| 巻・号 | 9(6) |
| ページ | e100495 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0100495 |
| PII | PONE-D-14-17661 |
| PMID | 24960176 |
| PMC | PMC4069002 |
| MeSH | Apoptosis / drug effects* CDC2 Protein Kinase Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / metabolism* Cell Cycle Proteins / antagonists & inhibitors* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Line, Tumor Cyclin-Dependent Kinases / metabolism Enzyme Activation / drug effects Gene Expression Humans Immunohistochemistry Liver Neoplasms / genetics Liver Neoplasms / metabolism* Nuclear Proteins / antagonists & inhibitors* Nuclear Proteins / genetics Nuclear Proteins / metabolism Protein Kinase Inhibitors / pharmacology* Protein-Tyrosine Kinases / antagonists & inhibitors* Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / metabolism RNA Interference Transforming Growth Factor beta1 / pharmacology |
| IF | 2.74 |
| 引用数 | 16 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7 |