論文 - 詳細
| RRC ID | 45604 |
|---|---|
| 著者 | Gengyo-Ando K, Mitani S. |
| タイトル | Characterization of mutations induced by ethyl methanesulfonate, UV, and trimethylpsoralen in the nematode Caenorhabditis elegans. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The genome project of the nematode Caenorhabditis elegans is completed. It is important and useful to disrupt nematode genes to know their function. We treated wild-type animals with potential candidates for mutagens for reverse genetics, EMS (ethyl methanesulfonate), short-wavelength UV, and long-wavelength UV in the presence of TMP (trimethylpsoralen). We estimated forward mutation rates by counting the occurrence of a marker unc-22 mutation. We found that the forward mutation rate by TMP/UV could be comparable with EMS by improving the frequency one order higher than before. We next isolated mutants of another marker gene ben-1 and examined the probability for the deletion mutations by PCR and sequencing. Deletion mutations were found only by TMP/UV method, which suggested TMP/UV is the choice for deletion mutagenesis among these methods. As a pilot experiment, we could isolate actual deletion mutations at a much higher frequency than previously. |
| 巻・号 | 269(1) |
| ページ | 64-9 |
| 公開日 | 2000-3-5 |
| DOI | 10.1006/bbrc.2000.2260 |
| PII | S0006-291X(00)92260-5 |
| PMID | 10694478 |
| MeSH | Animals Base Sequence Caenorhabditis elegans / drug effects Caenorhabditis elegans / genetics* Caenorhabditis elegans / radiation effects DNA Primers / genetics DNA, Helminth / genetics Ethyl Methanesulfonate / toxicity Genes, Helminth / drug effects Genes, Helminth / radiation effects Genetic Markers Mutation* Phenotype Sequence Deletion Trioxsalen / toxicity Ultraviolet Rays / adverse effects |
| IF | 2.985 |
| 引用数 | 152 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm25 tm124 tm177 tm234 |