Reference - Detail
RRC ID | 45628 |
---|---|
Author | Prevo B, Mangeol P, Oswald F, Scholey JM, Peterman EJ. |
Title | Functional differentiation of cooperating kinesin-2 motors orchestrates cargo import and transport in C. elegans cilia. |
Journal | Nat Cell Biol |
Abstract |
Intracellular transport depends on cooperation between distinct motor proteins. Two anterograde intraflagellar transport (IFT) motors, heterotrimeric kinesin-II and homodimeric OSM-3, cooperate to move cargo along Caenorhabditis elegans cilia. Here, using quantitative fluorescence microscopy, with single-molecule sensitivity, of IFT in living strains containing single-copy transgenes encoding fluorescent IFT proteins, we show that kinesin-II transports IFT trains through the ciliary base and transition zone to a 'handover zone' on the proximal axoneme. There, OSM-3 gradually replaces kinesin-II, yielding velocity profiles inconsistent with in vitro motility assays, and then drives transport to the ciliary tip. Dissociated kinesin-II motors undergo rapid turnaround and recycling to the ciliary base, whereas OSM-3 is recycled mainly to the handover zone. This reveals a functional differentiation in which the slower, less processive kinesin-II imports IFT trains into the cilium and OSM-3 drives their long-range transport, thereby optimizing cargo delivery. |
Volume | 17(12) |
Pages | 1536-45 |
Published | 2015-12-1 |
DOI | 10.1038/ncb3263 |
PII | ncb3263 |
PMID | 26523365 |
MeSH | A Kinase Anchor Proteins / genetics A Kinase Anchor Proteins / metabolism Algorithms Animals Biological Transport / genetics Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cilia / metabolism* Computer Simulation Kinesins / chemistry Kinesins / genetics Kinesins / metabolism* Kinetics Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Fluorescence Models, Biological Mutation Protein Multimerization Video Recording |
IF | 20.042 |
Times Cited | 70 |
WOS Category | CELL BIOLOGY |
Resource | |
C.elegans | tm3433 |