論文 - 詳細
| RRC ID | 45647 |
|---|---|
| 著者 | Tang L, Choe KP. |
| タイトル | Characterization of skn-1/wdr-23 phenotypes in Caenorhabditis elegans; pleiotrophy, aging, glutathione, and interactions with other longevity pathways. |
| ジャーナル | Mech Ageing Dev |
| Abstract |
The SKN-1/Nrf transcription factors are master regulators of oxidative stress responses and are emerging as important determinants of longevity. We previously identified a protein named WDR-23 as a direct repressor of SKN-1 in C. elegans. Loss of wdr-23 influences stress resistance, longevity, development, and reproduction, but it is unknown if WDR-23 influences development and reproduction solely through SKN-1 and the mechanisms by which SKN-1 promotes stress resistance and longevity are poorly defined. Here, we characterize phenotypes of wdr-23 and skn-1 manipulation and explore the role of glutathione. We provide evidence that diverse wdr-23 phenotypes are dependent on SKN-1, that beneficial and detrimental phenotypes of wdr-23 and skn-1 can be partially decoupled, and that SKN-1 activation delays degenerative tissue changes during aging. We also show that total glutathione levels are substantially elevated when the wdr-23/skn-1 pathway is activated and that skn-1 is required for preserving this cellular antioxidant during stress and aging. Alternatively, total glutathione was not elevated in worms with reduced insulin/IGF-1-like signaling or dietary restriction suggesting that SKN-1 ensures longevity via different mechanisms under these conditions. Lastly, genetic interaction data revise our understanding of which skn-1 variants are required for longevity during dietary restriction. |
| 巻・号 | 149 |
| ページ | 88-98 |
| 公開日 | 2015-7-1 |
| DOI | 10.1016/j.mad.2015.06.001 |
| PII | S0047-6374(15)00080-9 |
| PMID | 26056713 |
| MeSH | Animals Arsenites / chemistry Body Size Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation Glutathione / metabolism* Longevity* Mutation Phenotype Pigmentation RNA Interference Repressor Proteins / genetics Repressor Proteins / metabolism* Signal Transduction Time Factors Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 4.304 |
| 引用数 | 22 |
| WOS 分野 | GERIATRICS & GERONTOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | NA |