RRC ID 45764
著者 Kasper DM, Wang G, Gardner KE, Johnstone TG, Reinke V.
タイトル The C. elegans SNAPc component SNPC-4 coats piRNA domains and is globally required for piRNA abundance.
ジャーナル Dev Cell
Abstract The Piwi/Piwi-interacting RNA (piRNA) pathway protects the germline from the activity of foreign sequences such as transposons. Remarkably, tens of thousands of piRNAs arise from a minimal number of discrete genomic regions. The extent to which clustering of these small RNA genes contributes to their coordinated expression remains unclear. We show that C. elegans SNPC-4, the Myb-like DNA-binding subunit of the small nuclear RNA activating protein complex, binds piRNA clusters in a germline-specific manner and is required for global piRNA expression. SNPC-4 localization is mutually dependent with localization of piRNA biogenesis factor PRDE-1. SNPC-4 exhibits an atypical widely distributed binding pattern that "coats" piRNA domains. Discrete peaks within the domains occur frequently at RNA-polymerase-III-occupied transfer RNA (tRNA) genes, which have been implicated in chromatin organization. We suggest that SNPC-4 binding establishes a positive expression environment across piRNA domains, providing an explanation for the conserved clustering of individually transcribed piRNA genes.
巻・号 31(2)
ページ 145-58
公開日 2014-10-27
DOI 10.1016/j.devcel.2014.09.015
PII S1534-5807(14)00621-2
PMID 25373775
PMC PMC4223638
MeSH Animals Base Sequence Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Cell Nucleus / metabolism Chromatin / genetics Chromosomal Proteins, Non-Histone / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Epigenesis, Genetic RNA Polymerase III / genetics RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* Sequence Analysis, RNA Signal Transduction / genetics Transcription Factors / genetics
IF 10.092
引用数 15
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm4568 tm1813. tm2303 tm0872