論文 - 詳細
| RRC ID | 45764 |
|---|---|
| 著者 | Kasper DM, Wang G, Gardner KE, Johnstone TG, Reinke V. |
| タイトル | The C. elegans SNAPc component SNPC-4 coats piRNA domains and is globally required for piRNA abundance. |
| ジャーナル | Dev Cell |
| Abstract |
The Piwi/Piwi-interacting RNA (piRNA) pathway protects the germline from the activity of foreign sequences such as transposons. Remarkably, tens of thousands of piRNAs arise from a minimal number of discrete genomic regions. The extent to which clustering of these small RNA genes contributes to their coordinated expression remains unclear. We show that C. elegans SNPC-4, the Myb-like DNA-binding subunit of the small nuclear RNA activating protein complex, binds piRNA clusters in a germline-specific manner and is required for global piRNA expression. SNPC-4 localization is mutually dependent with localization of piRNA biogenesis factor PRDE-1. SNPC-4 exhibits an atypical widely distributed binding pattern that "coats" piRNA domains. Discrete peaks within the domains occur frequently at RNA-polymerase-III-occupied transfer RNA (tRNA) genes, which have been implicated in chromatin organization. We suggest that SNPC-4 binding establishes a positive expression environment across piRNA domains, providing an explanation for the conserved clustering of individually transcribed piRNA genes. |
| 巻・号 | 31(2) |
| ページ | 145-58 |
| 公開日 | 2014-10-27 |
| DOI | 10.1016/j.devcel.2014.09.015 |
| PII | S1534-5807(14)00621-2 |
| PMID | 25373775 |
| PMC | PMC4223638 |
| MeSH | Animals Base Sequence Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Cell Nucleus / metabolism Chromatin / genetics Chromosomal Proteins, Non-Histone / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Epigenesis, Genetic RNA Polymerase III / genetics RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* Sequence Analysis, RNA Signal Transduction / genetics Transcription Factors / genetics |
| IF | 10.092 |
| 引用数 | 15 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4568 tm1813. tm2303 tm0872 |