論文 - 詳細
| RRC ID | 45802 |
|---|---|
| 著者 | Murakami-Tonami Y, Ikeda H, Yamagishi R, Inayoshi M, Inagaki S, Kishida S, Komata Y, Jan Koster, Takeuchi I, Kondo Y, Maeda T, Sekido Y, Murakami H, Kadomatsu K. |
| タイトル | SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells. |
| ジャーナル | Sci Rep |
| Abstract |
Shugoshin 1 (SGO1) is required for accurate chromosome segregation during mitosis and meiosis; however, its other functions, especially at interphase, are not clearly understood. Here, we found that downregulation of SGO1 caused a synergistic phenotype in cells overexpressing MYCN. Downregulation of SGO1 impaired proliferation and induced DNA damage followed by a senescence-like phenotype only in MYCN-overexpressing neuroblastoma cells. In these cells, SGO1 knockdown induced DNA damage, even during interphase, and this effect was independent of cohesin. Furthermore, MYCN-promoted SGO1 transcription and SGO1 expression tended to be higher in MYCN- or MYC-overexpressing cancers. Together, these findings indicate that SGO1 plays a role in the DNA damage response in interphase. Therefore, we propose that SGO1 represents a potential molecular target for treatment of MYCN-amplified neuroblastoma. |
| 巻・号 | 6 |
| ページ | 31615 |
| 公開日 | 2016-8-19 |
| DOI | 10.1038/srep31615 |
| PII | srep31615 |
| PMID | 27539729 |
| PMC | PMC4990925 |
| MeSH | Animals Cell Cycle Proteins / biosynthesis* Cell Cycle Proteins / genetics Cell Line, Tumor DNA Damage* Gene Amplification* Gene Expression Regulation, Neoplastic* Interphase / genetics Mice Mice, Transgenic N-Myc Proto-Oncogene Protein / genetics N-Myc Proto-Oncogene Protein / metabolism* Neuroblastoma / genetics Neuroblastoma / metabolism* Neuroblastoma / pathology Transcription, Genetic* |
| IF | 3.998 |
| 引用数 | 6 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-CMV-RfA-IRES2-Venus (RDB04388) CSII-CMV-Venus (RDB05553) CSII-CMV-mRFP (RDB06386). |