RRC ID 45805
Author Shikata H, Yakushijin Y, Matsushita N, Sakai A, Sugita A, Nakamura N, Yamanouchi J, Azuma T, Hato T, Yasukawa M.
Title Role of activation-induced cytidine deaminase in the progression of follicular lymphoma.
Journal Cancer Sci
Abstract Activation-induced cytidine deaminase (AID/AICDA) is required for somatic hypermutation and class-switch recombination of the immunoglobulin gene, and for c-myc translocation of germinal center-derived B-cell lymphoma. In the present study, we attempted to clarify the significance of AID associated with c-myc in the progression of follicular lymphoma (FL) using RT-PCR and quantitative real-time PCR. Tissues from the patients with grade 3 FL expressed relatively higher levels of c-myc and AID. The samples taken from a patient with FL who died within 2 years after the start of treatment showed either no or low expression of AID, despite expressing high levels of c-myc. In order to examine the role of AID expression in rapidly progressive FL, the full-length AID transcript was transfected into AID-negative cell lines established from different patients with rapidly progressive FL. This led to the establishment of AID-expressing transfectants with a low proliferation rate and a significantly increased incidence of G(0)/G(1) arrest compared with controls. Our results indicate that AID may act as a negative regulator of cell survival in FL when sufficient c-myc is expressed. Switch-off or low expression of AID after c-myc amplification may correlate with the clinical outcomes of FL.
Volume 103(3)
Pages 415-21
Published 2012-3-1
DOI 10.1111/j.1349-7006.2011.02186.x
PMID 22168746
PMC PMC7713616
MeSH Adult Aged Blotting, Western Cell Proliferation Cytidine Deaminase / genetics Cytidine Deaminase / metabolism* Disease Progression Enzyme Activation Female Gene Expression Profiling Humans Immunohistochemistry Lymphoma, Follicular / genetics Lymphoma, Follicular / metabolism* Lymphoma, Follicular / pathology Male Middle Aged Proto-Oncogene Proteins c-myc / genetics Proto-Oncogene Proteins c-myc / metabolism* Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Transfection
IF 4.966
Times Cited 8
DNA material CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393).