RRC ID 45833
Author Qin J, Liang J, Ding M.
Title Perlecan antagonizes collagen IV and ADAMTS9/GON-1 in restricting the growth of presynaptic boutons.
Journal J Neurosci
Abstract In the mature nervous system, a significant fraction of synapses are structurally stable over a long time scale. However, the mechanisms that restrict synaptic growth within a confined region are poorly understood. Here, we identified that in the C. elegans neuromuscular junction, collagens Type IV and XVIII, and the secreted metalloprotease ADAMTS/GON-1 are critical for growth restriction of presynaptic boutons. Without these components, ectopic boutons progressively invade into the nonsynaptic region. Perlecan/UNC-52 promotes the growth of ectopic boutons and functions antagonistically to collagen Type IV and GON-1 but not to collagen XVIII. The growth constraint of presynaptic boutons correlates with the integrity of the extracellular matrix basal lamina or basement membrane (BM), which surrounds chemical synapses. Fragmented BM appears in the region where ectopic boutons emerge. Further removal of UNC-52 improves the BM integrity and the tight association between BM and presynaptic boutons. Together, our results unravel the complex role of the BM in restricting the growth of presynaptic boutons and reveal the antagonistic function of perlecan on Type IV collagen and ADAMTS protein.
Volume 34(31)
Pages 10311-24
Published 2014-7-30
DOI 10.1523/JNEUROSCI.5128-13.2014
PII 34/31/10311
PMID 25080592
PMC PMC6608278
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Collagen Type IV / metabolism* Gene Expression Regulation, Developmental / genetics Heparan Sulfate Proteoglycans / genetics Heparan Sulfate Proteoglycans / metabolism* Imaging, Three-Dimensional Luminescent Proteins / genetics Male Metalloendopeptidases / genetics Metalloendopeptidases / metabolism* Microscopy, Electron, Transmission Neuromuscular Junction / cytology* Optical Imaging Presynaptic Terminals / metabolism* Presynaptic Terminals / ultrastructure* RNA Interference / physiology
IF 5.674
Times Cited 15
WOS Category NEUROSCIENCES
Resource
C.elegans tm1092