| RRC ID |
45969
|
| 著者 |
Fischer SE, Pan Q, Breen PC, Qi Y, Shi Z, Zhang C, Ruvkun G.
|
| タイトル |
Multiple small RNA pathways regulate the silencing of repeated and foreign genes in C. elegans.
|
| ジャーナル |
Genes Dev
|
| Abstract |
Gene segments from other organisms, such as viruses, are detected as foreign and targeted for silencing by RNAi pathways. A deep-sequencing map of the small RNA response to repeated transgenes introduced to Caenorhabditis elegans revealed that specific segments are targeted by siRNAs. Silencing of the foreign gene segments depends on an antiviral response that involves changes in active and silent chromatin modifications and altered levels of antisense siRNAs. Distinct Argonaute proteins target foreign genes for silencing or protection against silencing. We used a repeated transgene in a genome-wide screen to identify gene disruptions that enhance silencing of foreign genetic elements and identified 69 genes. These genes cluster in four groups based on overlapping sets of coexpressed genes, including a group of germline-expressed genes that are likely coregulated by the E2F transcription factor. Many of the gene inactivations enhance exogenous RNAi. About half of the 69 genes have roles in endogenous RNAi pathways that regulate diverse processes, including silencing of duplicated genes and transposons and chromosome segregation. Of these newly identified genes, several are required for siRNA biogenesis or stability in the oocyte-specific ERGO-1 pathway, including eri-12, encoding an interactor of the RNAi-defective protein RDE-10, and ntl-9/CNOT9, one of several CCR4/NOT complex genes that we identified. The conserved ARF-like small GTPase ARL-8 is required specifically for primary siRNA biogenesis or stability in the sperm-specific ALG-3/4 endogenous RNAi pathway.
|
| 巻・号 |
27(24)
|
| ページ |
2678-95
|
| 公開日 |
2013-12-15
|
| DOI |
10.1101/gad.233254.113
|
| PII |
27/24/2678
|
| PMID |
24352423
|
| PMC |
PMC3877757
|
| MeSH |
Animals
Argonaute Proteins / metabolism
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Chromatin / metabolism
Gene Expression Regulation
Gene Silencing / physiology*
Genes, Viral / genetics
Genome, Helminth / genetics
Humans
Multigene Family
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism*
Transgenes / genetics
|
| IF |
9.527
|
| 引用数 |
10
|
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2472
tm2388 |
