RRC ID 46008
著者 Al Rawi S, Louvet-Vallée S, Djeddi A, Sachse M, Culetto E, Hajjar C, Boyd L, Legouis R, Galy V.
タイトル Postfertilization autophagy of sperm organelles prevents paternal mitochondrial DNA transmission.
ジャーナル Science
Abstract In sexual reproduction of most animals, the spermatozoon provides DNA and centrioles, together with some cytoplasm and organelles, to the oocyte that is being fertilized. Paternal mitochondria and their genomes are generally eliminated in the embryo by an unknown degradation mechanism. We show that, upon fertilization, a Caenorhabditis elegans spermatozoon triggers the recruitment of autophagosomes within minutes and subsequent paternal mitochondria degradation. Whereas the nematode-specific sperm membranous organelles are ubiquitinated before autophagosome formation, the mitochondria are not. The degradation of both paternal structures and mitochondrial DNA requires an LC3-dependent autophagy. Analysis of fertilized mouse embryos shows the localization of autophagy markers, which suggests that this autophagy event is evolutionarily conserved to prevent both the transmission of paternal mitochondrial DNA to the offspring and the establishment of heteroplasmy.
巻・号 334(6059)
ページ 1144-7
公開日 2011-11-25
DOI 10.1126/science.1211878
PII science.1211878
PMID 22033522
MeSH Animals Autophagy* Caenorhabditis elegans / embryology* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / analysis DNA, Mitochondrial / analysis DNA, Mitochondrial / genetics* DNA, Mitochondrial / metabolism Embryo, Nonmammalian / physiology* Embryonic Development Female Fertilization Hermaphroditic Organisms Lysosomes / metabolism Male Mice Mitochondria / metabolism* Oocytes / physiology Organelles / metabolism Phagosomes / metabolism Spermatozoa / chemistry Spermatozoa / physiology Spermatozoa / ultrastructure* Ubiquitination
IF 41.846
引用数 248
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm3489