RRC ID 46083
著者 Marcette JD, Chen JJ, Nonet ML.
タイトル The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites.
ジャーナル Elife
Abstract Microtubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored. CAMSAP homologs associate with microtubule minus-ends, and are important for the stability of epithelial cell adhesions. In this study, we report morphological defects in neurons and neuromuscular defects in mutants of the C. elegans CAMSAP, ptrn-1. Mechanosensory neurons initially extend wild-type neurites, and subsequently remodel by overextending neurites and retracting synaptic branches and presynaptic varicosities. This neuronal remodeling can be activated by mutations known to alter microtubules, and depends on a functioning DLK-1 MAP kinase pathway. We found that PTRN-1 localizes to both neurites and synapses, and our results suggest that alterations of microtubule structures caused by loss of PTRN-1 function activates a remodeling program leading to changes in neurite morphology. We propose a model whereby minus-end microtubule stabilization mediated by a functional PTRN-1 is necessary for morphological maintenance of neurons. DOI: http://dx.doi.org/10.7554/eLife.01637.001.
巻・号 3
ページ e01637
公開日 2014-2-25
DOI 10.7554/eLife.01637
PMID 24569480
PMC PMC3930908
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Shape Electrical Synapses / metabolism* Gene Expression Regulation Genotype Locomotion MAP Kinase Kinase Kinases / genetics MAP Kinase Kinase Kinases / metabolism Mechanotransduction, Cellular Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Mutation Neurites / metabolism* Phenotype Presynaptic Terminals / metabolism* Protein Stability
IF 7.08
引用数 44
WOS 分野 BIOLOGY
リソース情報
線虫 tm5597