論文 - 詳細
| RRC ID | 46083 |
|---|---|
| 著者 | Marcette JD, Chen JJ, Nonet ML. |
| タイトル | The Caenorhabditis elegans microtubule minus-end binding homolog PTRN-1 stabilizes synapses and neurites. |
| ジャーナル | Elife |
| Abstract |
Microtubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored. CAMSAP homologs associate with microtubule minus-ends, and are important for the stability of epithelial cell adhesions. In this study, we report morphological defects in neurons and neuromuscular defects in mutants of the C. elegans CAMSAP, ptrn-1. Mechanosensory neurons initially extend wild-type neurites, and subsequently remodel by overextending neurites and retracting synaptic branches and presynaptic varicosities. This neuronal remodeling can be activated by mutations known to alter microtubules, and depends on a functioning DLK-1 MAP kinase pathway. We found that PTRN-1 localizes to both neurites and synapses, and our results suggest that alterations of microtubule structures caused by loss of PTRN-1 function activates a remodeling program leading to changes in neurite morphology. We propose a model whereby minus-end microtubule stabilization mediated by a functional PTRN-1 is necessary for morphological maintenance of neurons. DOI: http://dx.doi.org/10.7554/eLife.01637.001. |
| 巻・号 | 3 |
| ページ | e01637 |
| 公開日 | 2014-2-25 |
| DOI | 10.7554/eLife.01637 |
| PMID | 24569480 |
| PMC | PMC3930908 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Shape Electrical Synapses / metabolism* Gene Expression Regulation Genotype Locomotion MAP Kinase Kinase Kinases / genetics MAP Kinase Kinase Kinases / metabolism Mechanotransduction, Cellular Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Mutation Neurites / metabolism* Phenotype Presynaptic Terminals / metabolism* Protein Stability |
| IF | 7.08 |
| 引用数 | 44 |
| WOS 分野 | BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm5597 |