Reference - Detail
RRC ID | 46145 |
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Author | Kovacevic I, Ho R, Cram EJ. |
Title | CCDC-55 is required for larval development and distal tip cell migration in Caenorhabditis elegans. |
Journal | Mech Dev |
Abstract |
The Caenorhabditis elegans distal tip cells (DTCs) are an in vivo model for the study of developmentally regulated cell migration. In this study, we characterize a novel role for CCDC-55, a conserved coiled-coil domain containing protein, in DTC migration and larval development in C. elegans. Although animals homozygous for a probable null allele, ccdc-55(ok2851), display an early larval arrest, RNAi depletion experiments allow the analysis of later phenotypes and suggest that CCDC-55 is needed within the DTC for migration to cease at the end of larval morphogenesis. The ccdc-55 gene is found in an operon with rnf-121 and rnf-5, E3 ubiquitin ligases that target cell migration genes such as the β-integrin PAT-3. Genetic interaction studies using RNAi depletion and the deletion alleles rnf-121(ok848) and rnf-5(tm794) indicate that CCDC-55 and the RNF genes act at least partially in parallel to promote termination of cell migration in the adult DTC. |
Volume | 128(11-12) |
Pages | 548-59 |
Published | 2012-1-1 |
DOI | 10.1016/j.mod.2012.01.003 |
PII | S0925-4773(12)00004-4 |
PMID | 22285439 |
PMC | PMC3295921 |
MeSH | Alleles Amino Acid Sequence Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Cell Movement* Gene Expression Gene Knockdown Techniques Larva / cytology Larva / growth & development Molecular Sequence Data Morphogenesis Protein Structure, Tertiary RNA Interference Sequence Homology, Amino Acid Ubiquitin-Protein Ligases / metabolism |
IF | 2.126 |
Times Cited | 6 |
WOS Category | DEVELOPMENTAL BIOLOGY |
Resource | |
C.elegans | tm794 |