RRC ID 46165
Author Maures TJ, Greer EL, Hauswirth AG, Brunet A.
Title The H3K27 demethylase UTX-1 regulates C. elegans lifespan in a germline-independent, insulin-dependent manner.
Journal Aging Cell
Abstract Aging is accompanied by alterations in epigenetic marks that control chromatin states, including histone acetylation and methylation. Enzymes that reversibly affect histone marks associated with active chromatin have recently been found to regulate aging in Caenorhabditis elegans. However, relatively little is known about the importance for aging of histone marks associated with repressed chromatin. Here, we use a targeted RNAi screen in C. elegans to identify four histone demethylases that significantly regulate worm lifespan, UTX-1, RBR-2, LSD-1, and T26A5.5. Interestingly, UTX-1 belongs to a conserved family of histone demethylases specific for lysine 27 of histone H3 (H3K27me3), a mark associated with repressed chromatin. Both utx-1 knockdown and heterozygous mutation of utx-1 extend lifespan and increase the global levels of the H3K27me3 mark in worms. The H3K27me3 mark significantly drops in somatic cells during the normal aging process. UTX-1 regulates lifespan independently of the presence of the germline, but in a manner that depends on the insulin-FoxO signaling pathway. These findings identify the H3K27me3 histone demethylase UTX-1 as a novel regulator of worm lifespan in somatic cells.
Volume 10(6)
Pages 980-90
Published 2011-12-1
DOI 10.1111/j.1474-9726.2011.00738.x
PMID 21834846
PMC PMC3215905
MeSH Animals Biomarkers / metabolism Blotting, Western Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Chromatin / genetics Chromatin / metabolism* Gene Expression Regulation* Gene Knockdown Techniques Germ Cells / metabolism High-Throughput Screening Assays Histone Demethylases / genetics Histone Demethylases / metabolism* Histones / genetics Histones / metabolism* Insulin / metabolism Longevity* Methylation Polymerase Chain Reaction RNA Interference Signal Transduction / genetics* Transcription Factors / genetics Transcription Factors / metabolism*
IF 7.238
Times Cited 125
C.elegans tm3118