Reference - Detail
RRC ID | 46172 |
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Author | Richardson CE, Spilker KA, Cueva JG, Perrino J, Goodman MB, Shen K. |
Title | PTRN-1, a microtubule minus end-binding CAMSAP homolog, promotes microtubule function in Caenorhabditis elegans neurons. |
Journal | Elife |
Abstract |
In neuronal processes, microtubules (MTs) provide structural support and serve as tracks for molecular motors. While it is known that neuronal MTs are more stable than MTs in non-neuronal cells, the molecular mechanisms underlying this stability are not fully understood. In this study, we used live fluorescence microscopy to show that the C. elegans CAMSAP protein PTRN-1 localizes to puncta along neuronal processes, stabilizes MT foci, and promotes MT polymerization in neurites. Electron microscopy revealed that ptrn-1 null mutants have fewer MTs and abnormal MT organization in the PLM neuron. Animals grown with a MT depolymerizing drug caused synthetic defects in neurite branching in the absence of ptrn-1 function, indicating that PTRN-1 promotes MT stability. Further, ptrn-1 null mutants exhibited aberrant neurite morphology and synaptic vesicle localization that is partially dependent on dlk-1. Our results suggest that PTRN-1 represents an important mechanism for promoting MT stability in neurons. DOI: http://dx.doi.org/10.7554/eLife.01498.001. |
Volume | 3 |
Pages | e01498 |
Published | 2014-2-25 |
DOI | 10.7554/eLife.01498 |
PMID | 24569477 |
PMC | PMC3932522 |
MeSH | Animals Caenorhabditis elegans / drug effects Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Genotype MAP Kinase Kinase Kinases / metabolism Microscopy, Electron Microscopy, Fluorescence Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Microtubules / drug effects Microtubules / metabolism* Mutation Neurites / metabolism Neurons / drug effects Neurons / metabolism* Phenotype Signal Transduction Synaptic Vesicles / metabolism Tubulin Modulators / pharmacology |
IF | 7.08 |
Times Cited | 51 |
WOS Category | BIOLOGY |
Resource | |
C.elegans | tm5597 |