論文 - 詳細
| RRC ID | 46183 |
|---|---|
| 著者 | Safratowich BD, Hossain M, Bianchi L, Carvelli L. |
| タイトル | Amphetamine potentiates the effects of β-phenylethylamine through activation of an amine-gated chloride channel. |
| ジャーナル | J Neurosci |
| Abstract |
β-Phenylethylamine (βPEA) is a trace amine present in the CNS of all animals tested to date. However, its function is still not fully understood. βPEA has been suggested to function as a neurotransmitter and/or to mimic the effect of amphetamine (Amph). In support of the latter is the observation that βPEA and Amph produce similar but not identical behaviors. Here, we show that βPEA, like Amph, activates the dopamine transporter and the amine-gated chloride channel LGC-55 to generate behaviors in Caenorhabditis elegans. However, although Amph-induced behaviors occurred gradually during 10 min of treatment, βPEA induced maximal effects within 1 min. In vitro data demonstrate that βPEA activates the LGC-55 more efficiently than Amph (Km = 9 and 152 μm, respectively) and generates saturating currents that are 10 times larger than those produced by Amph. These results suggest that activation of LGC-55 mostly accounts for the behavioral effects reached after 1 min of treatment with βPEA. Importantly, our in vitro and in vivo data show that Amph increases the effects induced by βPEA on the LGC-55, indicating that Amph potentiates the effects generated by the biogenic amine βPEA. Together, our data not only identify a new target for βPEA, but also offer a novel mechanism of action of Amph. In addition, our results highlight C. elegans as a powerful genetic model for studying the effects of biogenic and synthetic amines both at the molecular and behavioral levels. |
| 巻・号 | 34(13) |
| ページ | 4686-91 |
| 公開日 | 2014-3-26 |
| DOI | 10.1523/JNEUROSCI.3100-13.2014 |
| PII | 34/13/4686 |
| PMID | 24672014 |
| PMC | PMC3965791 |
| MeSH | Amines / metabolism Amines / pharmacology* Amphetamine / pharmacology* Animals Animals, Genetically Modified Caenorhabditis elegans / drug effects Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Cells, Cultured Central Nervous System Stimulants / pharmacology* Chloride Channels / genetics Chloride Channels / metabolism* Dopamine / metabolism Dopamine Plasma Membrane Transport Proteins / genetics Dopamine Plasma Membrane Transport Proteins / metabolism Dose-Response Relationship, Drug Membrane Potentials / drug effects Membrane Potentials / genetics Oocytes Phenethylamines / pharmacology* Psychotropic Drugs / pharmacology* Receptors, Biogenic Amine Receptors, Dopamine / metabolism |
| IF | 5.674 |
| 引用数 | 12 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1392 tm2913 |