RRC ID 46189
Author Seth M, Shirayama M, Gu W, Ishidate T, Conte D Jr, Mello CC.
Title The C. elegans CSR-1 argonaute pathway counteracts epigenetic silencing to promote germline gene expression.
Journal Dev. Cell
Abstract Organisms can develop adaptive sequence-specific immunity by reexpressing pathogen-specific small RNAs that guide gene silencing. For example, the C. elegans PIWI-Argonaute/piwi-interacting RNA (piRNA) pathway recruits RNA-dependent RNA polymerase (RdRP) to foreign sequences to amplify a transgenerational small-RNA-induced epigenetic silencing signal (termed RNAe). Here, we provide evidence that, in addition to an adaptive memory of silenced sequences, C. elegans can also develop an opposing adaptive memory of expressed/self-mRNAs. We refer to this mechanism, which can prevent or reverse RNAe, as RNA-induced epigenetic gene activation (RNAa). We show that CSR-1, which engages RdRP-amplified small RNAs complementary to germline-expressed mRNAs, is required for RNAa. We show that a transgene with RNAa activity also exhibits accumulation of cognate CSR-1 small RNAs. Our findings suggest that C. elegans adaptively acquires and maintains a transgenerational CSR-1 memory that recognizes and protects self-mRNAs, allowing piRNAs to recognize foreign sequences innately, without the need for prior exposure
Volume 27(6)
Pages 656-63
Published 2013-12-23
DOI 10.1016/j.devcel.2013.11.014
PII S1534-5807(13)00695-3
PMID 24360782
PMC PMC3954781
MeSH Animals Argonaute Proteins / genetics Argonaute Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Epigenesis, Genetic* Gene Silencing* Germ Cells / metabolism* RNA Replicase / genetics RNA Replicase / metabolism RNA, Helminth / genetics* RNA, Helminth / metabolism RNA, Small Interfering / genetics RNA, Small Interfering / metabolism* Signal Transduction
IF 9.616
Times Cited 64
WOS Category DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
C.elegans tm892 tm872