RRC ID 46198
著者 Silva-García CG, Estela Navarro R.
タイトル The C. elegans TIA-1/TIAR homolog TIAR-1 is required to induce germ cell apoptosis.
ジャーナル Genesis
Abstract In Caenorhabditis elegans, physiological germ cell apoptosis eliminates more than half of the cells in the hermaphrodite gonad to support gamete quality and germline homeostasis by a still unidentified mechanism. External factors can also affect germ cell apoptosis. The BH3-only protein EGL-1 induces germ cell apoptosis when animals are exposed to pathogens or agents that produce DNA damage. DNA damage-induced apoptosis also requires the nematode p53 homolog CEP-1. Previously, we found that heat shock, oxidative, and osmotic stresses induce germ cell apoptosis through an EGL-1 and CEP-1 independent mechanism that requires the MAPKK pathway. However, we observed that starvation increases germ cell apoptosis by an unknown pathway. Searching for proteins that participate in stress-induced apoptosis, we found the RNA-binding protein TIAR-1 (a homolog of the mammalian TIA-1/TIAR family of proteins). Here, we show that TIAR-1 in C. elegans is required to induce apoptosis in the germline under several conditions. We also show that TIAR-1 acts downstream of CED-9 (a BCL2 homolog) to induce apoptosis under stress conditions, and apparently does not seem to regulate ced-4 or ced-3 mRNAs accumulation directly. TIAR-1 is expressed ubiquitously in the cytoplasm of the soma as well as the germline, where it sometimes associates with P granules. We show that animals lacking TIAR-1 expression are temperature sensitive sterile due to oogenesis and spermatogenesis defects. Our work shows that TIAR-1 is required for proper germline function and demonstrates that this protein is important to induce germ cell apoptosis under several conditions.
巻・号 51(10)
ページ 690-707
公開日 2013-10-1
DOI 10.1002/dvg.22418
PMID 23913578
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Apoptosis* Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Embryo, Nonmammalian Female Fertility Germ Cells / physiology* Male Molecular Sequence Data Phylogeny Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / physiology RNA-Binding Proteins / chemistry RNA-Binding Proteins / physiology* Sequence Homology
IF 1.76
引用数 7
WOS 分野 GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm361 tm2923