論文 - 詳細
| RRC ID | 46198 |
|---|---|
| 著者 | Silva-García CG, Estela Navarro R. |
| タイトル | The C. elegans TIA-1/TIAR homolog TIAR-1 is required to induce germ cell apoptosis. |
| ジャーナル | Genesis |
| Abstract |
In Caenorhabditis elegans, physiological germ cell apoptosis eliminates more than half of the cells in the hermaphrodite gonad to support gamete quality and germline homeostasis by a still unidentified mechanism. External factors can also affect germ cell apoptosis. The BH3-only protein EGL-1 induces germ cell apoptosis when animals are exposed to pathogens or agents that produce DNA damage. DNA damage-induced apoptosis also requires the nematode p53 homolog CEP-1. Previously, we found that heat shock, oxidative, and osmotic stresses induce germ cell apoptosis through an EGL-1 and CEP-1 independent mechanism that requires the MAPKK pathway. However, we observed that starvation increases germ cell apoptosis by an unknown pathway. Searching for proteins that participate in stress-induced apoptosis, we found the RNA-binding protein TIAR-1 (a homolog of the mammalian TIA-1/TIAR family of proteins). Here, we show that TIAR-1 in C. elegans is required to induce apoptosis in the germline under several conditions. We also show that TIAR-1 acts downstream of CED-9 (a BCL2 homolog) to induce apoptosis under stress conditions, and apparently does not seem to regulate ced-4 or ced-3 mRNAs accumulation directly. TIAR-1 is expressed ubiquitously in the cytoplasm of the soma as well as the germline, where it sometimes associates with P granules. We show that animals lacking TIAR-1 expression are temperature sensitive sterile due to oogenesis and spermatogenesis defects. Our work shows that TIAR-1 is required for proper germline function and demonstrates that this protein is important to induce germ cell apoptosis under several conditions. |
| 巻・号 | 51(10) |
| ページ | 690-707 |
| 公開日 | 2013-10-1 |
| DOI | 10.1002/dvg.22418 |
| PMID | 23913578 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Apoptosis* Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Embryo, Nonmammalian Female Fertility Germ Cells / physiology* Male Molecular Sequence Data Phylogeny Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / physiology RNA-Binding Proteins / chemistry RNA-Binding Proteins / physiology* Sequence Homology |
| IF | 1.76 |
| 引用数 | 7 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm361 tm2923 |