RRC ID 46218
Author Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP.
Title Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system.
Journal Genetics
Abstract We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.
Volume 195(3)
Pages 1181-5
Published 2013-11-1
DOI 10.1534/genetics.113.156075
PII genetics.113.156075
PMID 23979579
PMC PMC3813848
MeSH Animals CRISPR-Cas Systems* Caenorhabditis elegans / genetics* DNA Breaks, Double-Stranded DNA, Helminth / genetics Gene Targeting Genetic Engineering Genome, Helminth* Homologous Recombination Mutagenesis Mutagenesis, Insertional RNA Editing / genetics
IF 4.015
Times Cited 84
C.elegans tm1791