RRC ID 46230
Author Wang Y, Alam T, Hill-Harfe K, Lopez AJ, Leung CK, Iribarne D, Bruggeman B, Miyamoto MM, Harfe BD, Choe KP.
Title Phylogenetic, expression, and functional analyses of anoctamin homologs in Caenorhabditis elegans.
Journal Am J Physiol Regul Integr Comp Physiol
Abstract Ca(2+)-activated Cl(-) channels (CaCCs) are critical to processes such as epithelial transport, membrane excitability, and signal transduction. Anoctamin, or TMEM16, is a family of 10 mammalian transmembrane proteins, 2 of which were recently shown to function as CaCCs. The functions of other family members have not been firmly established, and almost nothing is known about anoctamins in invertebrates. Therefore, we performed a phylogenetic analysis of anoctamins across the animal kingdom and examined the expression and function of anoctamins in the genetically tractable nematode Caenorhabditis elegans. Phylogenetic analyses support five anoctamin clades that are at least as old as the deuterostome/protosome ancestor. This includes a branch containing two Drosophila paralogs that group with mammalian ANO1 and ANO2, the two best characterized CaCCs. We identify two anoctamins in C. elegans (ANOH-1 and ANOH-2) that are also present in basal metazoans. The anoh-1 promoter is active in amphid sensory neurons that detect external chemical and nociceptive cues. Within amphid neurons, ANOH-1::GFP fusion protein is enriched within sensory cilia. RNA interference silencing of anoh-1 reduced avoidance of steep osmotic gradients without disrupting amphid cilia development, chemotaxis, or withdrawal from noxious stimuli, suggesting that ANOH-1 functions in a sensory mode-specific manner. The anoh-2 promoter is active in mechanoreceptive neurons and the spermatheca, but loss of anoh-2 had no effect on motility or brood size. Our study indicates that at least five anoctamin duplicates are evolutionarily ancient and suggests that sensory signaling may be a basal function of the anoctamin protein family.
Volume 305(11)
Pages R1376-89
Published 2013-12-1
DOI 10.1152/ajpregu.00303.2012
PII ajpregu.00303.2012
PMID 24049119
MeSH Animals Biological Transport / genetics Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Calcium / metabolism Chloride Channels / genetics Chloride Channels / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Neurons, Afferent / metabolism Phylogeny* Signal Transduction / genetics
IF 3.026
Times Cited 11
WOS Category PHYSIOLOGY
Resource
C.elegans tm4762 tm4796