論文 - 詳細
| RRC ID | 46241 |
|---|---|
| 著者 | Xu M, Choi EY, Paik YK. |
| タイトル | Mutation of the lbp-5 gene alters metabolic output in Caenorhabditis elegans. |
| ジャーナル | BMB Rep |
| Abstract |
Intracellular lipid-binding proteins (LBPs) impact fatty acid homeostasis in various ways, including fatty acid transport into mitochondria. However, the physiological consequences caused by mutations in genes encoding LBPs remain largely uncharacterized. Here, we explore the metabolic consequences of lbp-5 gene deficiency in terms of energy homeostasis in Caenorhabditis elegans. In addition to increased fat storage, which has previously been reported, deletion of lbp-5 attenuated mitochondrial membrane potential and increased reactive oxygen species levels. Biochemical measurement coupled to proteomic analysis of the lbp-5(tm1618) mutant revealed highly increased rates of glycolysis in this mutant. These differential expression profile data support a novel metabolic adaptation of C. elegans, in which glycolysis is activated to compensate for the energy shortage due to the insufficient mitochondrial β-oxidation of fatty acids in lbp-5 mutant worms. This report marks the first demonstration of a unique metabolic adaptation that is a consequence of LBP-5 deficiency in C. elegans. |
| 巻・号 | 47(1) |
| ページ | 15-20 |
| 公開日 | 2014-1-1 |
| DOI | 10.5483/bmbrep.2014.47.1.086 |
| PII | 2319 |
| PMID | 24195791 |
| PMC | PMC4163843 |
| MeSH | Animals Antimetabolites / pharmacology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Deoxyglucose / pharmacology Energy Metabolism / genetics* Fatty Acids / metabolism Glycolysis Lipid Peroxidation Membrane Potential, Mitochondrial / drug effects Mitochondria / metabolism Mutation RNA, Messenger / metabolism Reactive Oxygen Species / metabolism |
| IF | 3.167 |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 線虫 | tm1618 |