RRC ID 46241
著者 Xu M, Choi EY, Paik YK.
タイトル Mutation of the lbp-5 gene alters metabolic output in Caenorhabditis elegans.
ジャーナル BMB Rep
Abstract Intracellular lipid-binding proteins (LBPs) impact fatty acid homeostasis in various ways, including fatty acid transport into mitochondria. However, the physiological consequences caused by mutations in genes encoding LBPs remain largely uncharacterized. Here, we explore the metabolic consequences of lbp-5 gene deficiency in terms of energy homeostasis in Caenorhabditis elegans. In addition to increased fat storage, which has previously been reported, deletion of lbp-5 attenuated mitochondrial membrane potential and increased reactive oxygen species levels. Biochemical measurement coupled to proteomic analysis of the lbp-5(tm1618) mutant revealed highly increased rates of glycolysis in this mutant. These differential expression profile data support a novel metabolic adaptation of C. elegans, in which glycolysis is activated to compensate for the energy shortage due to the insufficient mitochondrial β-oxidation of fatty acids in lbp-5 mutant worms. This report marks the first demonstration of a unique metabolic adaptation that is a consequence of LBP-5 deficiency in C. elegans.
巻・号 47(1)
ページ 15-20
公開日 2014-1-1
DOI 10.5483/bmbrep.2014.47.1.086
PII 2319
PMID 24195791
PMC PMC4163843
MeSH Animals Antimetabolites / pharmacology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Deoxyglucose / pharmacology Energy Metabolism / genetics* Fatty Acids / metabolism Glycolysis Lipid Peroxidation Membrane Potential, Mitochondrial / drug effects Mitochondria / metabolism Mutation RNA, Messenger / metabolism Reactive Oxygen Species / metabolism
IF 3.167
引用数 3
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm1618