論文 - 詳細
| RRC ID | 46253 |
|---|---|
| 著者 | Sasaki A, Nakae I, Nagasawa M, Hashimoto K, Abe F, Saito K, Fukuyama M, Gengyo-Ando K, Mitani S, Katada T, Kontani K. |
| タイトル | Arl8/ARL-8 functions in apoptotic cell removal by mediating phagolysosome formation in Caenorhabditis elegans. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Efficient clearance of apoptotic cells by phagocytes is important for development, tissue homeostasis, and the prevention of autoimmune responses. Phagosomes containing apoptotic cells undergo acidification and mature from Rab5-positive early to Rab7-positive late stages. Phagosomes finally fuse with lysosomes to form phagolysosomes, which degrade apoptotic cells; however, the molecular mechanism underlying phagosome-lysosome fusion is not fully understood. Here we show that the Caenorhabditis elegans Arf-like small GTPase Arl8 (ARL-8) is involved in phagolysosome formation and is required for the efficient removal of apoptotic cells. Loss of function of arl-8 results in the accumulation of apoptotic germ cells. Both the engulfment of the apoptotic cells by surrounding somatic sheath cells and the phagosomal maturation from RAB-5- to RAB-7-positive stages occur in arl-8 mutants. However, the phagosomes fail to fuse with lysosomes in the arl-8 mutants, leading to the accumulation of RAB-7-positive phagosomes and the delayed degradation of apoptotic cells. ARL-8 localizes primarily to lysosomes and physically interacts with the homotypic fusion and protein sorting complex component VPS-41. Collectively our findings reveal that ARL-8 facilitates apoptotic cell removal in vivo by mediating phagosome-lysosome fusion during phagocytosis. |
| 巻・号 | 24(10) |
| ページ | 1584-92 |
| 公開日 | 2013-5-1 |
| DOI | 10.1091/mbc.E12-08-0628 |
| PII | mbc.E12-08-0628 |
| PMID | 23485564 |
| PMC | PMC3655818 |
| MeSH | Animals Apoptosis* Caenorhabditis elegans / cytology Caenorhabditis elegans / enzymology* Caenorhabditis elegans Proteins / physiology* GTP Phosphohydrolases / physiology* Germ Cells / physiology Gonads / cytology Gonads / enzymology Lysosomes / enzymology Phagosomes / enzymology* Protein Transport Time-Lapse Imaging Vesicular Transport Proteins / metabolism rab GTP-Binding Proteins / metabolism rab7 GTP-Binding Proteins |
| IF | 3.791 |
| 引用数 | 28 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm2504 |