RRC ID 46285
著者 Cha DS, Hollis SE, Datla US, Lee S, Ryu J, Jung HR, Kim E, Kim K, Lee M, Li C, Lee MH.
タイトル Differential subcellular localization of DNA topoisomerase-1 isoforms and their roles during Caenorhabditis elegans development.
ジャーナル Gene Expr Patterns
Abstract DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. Caenorhabditis elegans TOP-1 has two isoforms, TOP-1α and TOP-1β. TOP-1β is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that top-1 transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1α in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that C. elegans TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms.
巻・号 12(5-6)
ページ 189-95
公開日 2012-1-1
DOI 10.1016/j.gep.2012.03.002
PII S1567-133X(12)00026-9
PMID 22452997
MeSH Alternative Splicing Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Chromosome Segregation DNA Topoisomerases, Type I / analysis DNA Topoisomerases, Type I / metabolism* Gonads / embryology Gonads / metabolism Isoenzymes / analysis Isoenzymes / metabolism
IF 0.897
引用数 3
WOS 分野 GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm3126