論文 - 詳細
| RRC ID | 46285 |
|---|---|
| 著者 | Cha DS, Hollis SE, Datla US, Lee S, Ryu J, Jung HR, Kim E, Kim K, Lee M, Li C, Lee MH. |
| タイトル | Differential subcellular localization of DNA topoisomerase-1 isoforms and their roles during Caenorhabditis elegans development. |
| ジャーナル | Gene Expr Patterns |
| Abstract |
DNA topoisomerase-1 (TOP-1) resolves the topological problems associated with DNA replication, transcription and recombination by introducing temporary single-strand breaks in the DNA. Caenorhabditis elegans TOP-1 has two isoforms, TOP-1α and TOP-1β. TOP-1β is broadly localized to the nuclei of many cells at all developmental stages and concentrated in nucleoli in embryo gut and oogenic cells. However, TOP-1α is specifically localized to centrosomes, neuronal cells, excretory cells and chromosomes of germ cells in embryonic and larval stages. Reporter gene analysis also shows that top-1 transcription is highly activated in several sensory neurons, speculating the possible role of TOP-1α in neuronal development. From RNA interference (RNAi) experiments, we demonstrated that C. elegans TOP-1 is required for chromosomal segregation, germline proliferation and gonadal migration, which are all correlated with the expression and activity of TOP-1. Therefore, our findings may provide an insight into a new role of TOP-1 in development of multicellular organisms. |
| 巻・号 | 12(5-6) |
| ページ | 189-95 |
| 公開日 | 2012-1-1 |
| DOI | 10.1016/j.gep.2012.03.002 |
| PII | S1567-133X(12)00026-9 |
| PMID | 22452997 |
| MeSH | Alternative Splicing Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Chromosome Segregation DNA Topoisomerases, Type I / analysis DNA Topoisomerases, Type I / metabolism* Gonads / embryology Gonads / metabolism Isoenzymes / analysis Isoenzymes / metabolism |
| IF | 0.897 |
| 引用数 | 3 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3126 |