RRC ID 46294
Author Mondoux MA, Love DC, Ghosh SK, Fukushige T, Bond M, Weerasinghe GR, Hanover JA, Krause MW.
Title O-linked-N-acetylglucosamine cycling and insulin signaling are required for the glucose stress response in Caenorhabditis elegans.
Journal Genetics
Abstract In a variety of organisms, including worms, flies, and mammals, glucose homeostasis is maintained by insulin-like signaling in a robust network of opposing and complementary signaling pathways. The hexosamine signaling pathway, terminating in O-linked-N-acetylglucosamine (O-GlcNAc) cycling, is a key sensor of nutrient status and has been genetically linked to the regulation of insulin signaling in Caenorhabditis elegans. Here we demonstrate that O-GlcNAc cycling and insulin signaling are both essential components of the C. elegans response to glucose stress. A number of insulin-dependent processes were found to be sensitive to glucose stress, including fertility, reproductive timing, and dauer formation, yet each of these differed in their threshold of sensitivity to glucose excess. Our findings suggest that O-GlcNAc cycling and insulin signaling are both required for a robust and adaptable response to glucose stress, but these two pathways show complex and interdependent roles in the maintenance of glucose-insulin homeostasis.
Volume 188(2)
Pages 369-82
Published 2011-6-1
DOI 10.1534/genetics.111.126490
PII genetics.111.126490
PMID 21441213
PMC PMC3122314
MeSH Acetylglucosamine / metabolism* Animals Blotting, Western Caenorhabditis elegans / drug effects Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Carbohydrates / analysis Dose-Response Relationship, Drug Female Glucose / metabolism* Glucose / pharmacology Insulin / metabolism* Larva / drug effects Larva / genetics Larva / metabolism Lipids / analysis Male Mutation N-Acetylglucosaminyltransferases / genetics N-Acetylglucosaminyltransferases / metabolism Receptor, Insulin / genetics Receptor, Insulin / metabolism Reproduction / drug effects Signal Transduction* Stress, Physiological Time Factors beta-N-Acetylhexosaminidases / genetics beta-N-Acetylhexosaminidases / metabolism
IF 4.015
Times Cited 42
C.elegans tm1046