論文 - 詳細
| RRC ID | 46322 |
|---|---|
| 著者 | Rodrigues AJ, Neves-Carvalho A, Teixeira-Castro A, Rokka A, Corthals G, Logarinho E, Maciel P. |
| タイトル | Absence of ataxin-3 leads to enhanced stress response in C. elegans. |
| ジャーナル | PLoS One |
| Abstract |
Ataxin-3, the protein involved in Machado-Joseph disease, is able to bind ubiquitylated substrates and act as a deubiquitylating enzyme in vitro, and it has been involved in the modulation of protein degradation by the ubiquitin-proteasome pathway. C. elegans and mouse ataxin-3 knockout models are viable and without any obvious phenotype in a basal condition however their phenotype in stress situations has never been described.Considering the role of ataxin-3 in the protein degradation pathway, we analyzed the effects of heat shock, a known protein homeostasis stressor, in C. elegans ataxin-3 (ATX-3) knockout animals. We found that ATX-3 mutants have an exacerbated stress response and survive significantly better than wild type animals when subjected to a noxious heat shock stimulus. This increased thermotolerance of mutants was further enhanced by pre-exposure to a mild heat shock. At a molecular level, ATX-3 mutants have a distinct transcriptomic and proteomic profile with several molecular chaperones abnormally up-regulated during heat shock and recovery, consistent with the observed resistance phenotype.The improved thermotolerance in ATX-3 mutants is independent of heat shock factor 1, the maestro of the heat shock response, but fully dependent on DAF-16, a critical stress responsive transcription factor involved in longevity and stress resistance. We also show that the increased thermotolerance of ATX-3 mutants is mainly due to HSP-16.2, C12C8.1 and F44E5.5 given that the knockdown of these heat shock proteins using RNA interference causes the phenotype to revert. This report suggests that the absence of ATX-3 activates the DAF-16 pathway leading to an overexpression of molecular chaperones, which yields knockout animals with an improved capacity for dealing with deleterious stimuli. |
| 巻・号 | 6(4) |
| ページ | e18512 |
| 公開日 | 2011-4-19 |
| DOI | 10.1371/journal.pone.0018512 |
| PMID | 21526185 |
| PMC | PMC3079722 |
| MeSH | Animals Ataxin-3 Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism Cold Temperature Forkhead Transcription Factors Gene Expression Regulation Gene Knockout Techniques Heat-Shock Response / genetics Longevity Mice Molecular Chaperones / genetics Molecular Chaperones / metabolism Mutation / genetics Nerve Tissue Proteins / deficiency* Nerve Tissue Proteins / metabolism Proteomics RNA, Messenger / genetics RNA, Messenger / metabolism Stress, Physiological* / genetics Transcription Factors / metabolism |
| IF | 2.74 |
| 引用数 | 18 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm1698 |