論文 - 詳細
| RRC ID | 46381 |
|---|---|
| 著者 | Khan LA, Zhang H, Abraham N, Sun L, Fleming JT, Buechner M, Hall DH, Gobel V. |
| タイトル | Intracellular lumen extension requires ERM-1-dependent apical membrane expansion and AQP-8-mediated flux. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Many unicellular tubes such as capillaries form lumens intracellularly, a process that is not well understood. Here we show that the cortical membrane organizer ERM-1 is required to expand the intracellular apical/lumenal membrane and its actin undercoat during single-cell Caenorhabditis elegans excretory canal morphogenesis. We characterize AQP-8, identified in an ERM-1-overexpression (ERM-1[++]) suppressor screen, as a canalicular aquaporin that interacts with ERM-1 in lumen extension in a mercury-sensitive manner, implicating water-channel activity. AQP-8 is transiently recruited to the lumen by ERM-1, co-localizing in peri-lumenal cuffs interspaced along expanding canals. An ERM-1[++]-mediated increase in the number of lumen-associated canaliculi is reversed by AQP-8 depletion. We propose that the ERM-1/AQP-8 interaction propels lumen extension by translumenal flux, suggesting a direct morphogenetic effect of water-channel-regulated fluid pressure. |
| 巻・号 | 15(2) |
| ページ | 143-56 |
| 公開日 | 2013-2-1 |
| DOI | 10.1038/ncb2656 |
| PII | ncb2656 |
| PMID | 23334498 |
| PMC | PMC4091717 |
| MeSH | Actin Cytoskeleton / metabolism Animals Animals, Genetically Modified Aquaporins / genetics Aquaporins / metabolism* Caenorhabditis elegans / drug effects Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Membrane / drug effects Cell Membrane / metabolism* Cell Membrane Permeability Cytoskeletal Proteins / genetics Cytoskeletal Proteins / metabolism* Gene Expression Regulation, Developmental Genotype Mercuric Chloride / pharmacology Morphogenesis Mutation Osmotic Pressure Phenotype Protein Binding Protein Transport RNA Interference Time Factors Water / metabolism Water-Electrolyte Balance |
| IF | 20.042 |
| 引用数 | 46 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm677 tm1919 |