Reference - Detail
| RRC ID | 46405 |
|---|---|
| Author | McNally KL, Fabritius AS, Ellefson ML, Flynn JR, Milan JA, McNally FJ. |
| Title | Kinesin-1 prevents capture of the oocyte meiotic spindle by the sperm aster. |
| Journal | Dev Cell |
| Abstract |
Centrioles are lost during oogenesis and inherited from the sperm at fertilization. In the zygote, the centrioles recruit pericentriolar proteins from the egg to form a mature centrosome that nucleates a sperm aster. The sperm aster then captures the female pronucleus to join the maternal and paternal genomes. Because fertilization occurs before completion of female meiosis, some mechanism must prevent capture of the meiotic spindle by the sperm aster. Here we show that in wild-type Caenorhabditis elegans zygotes, maternal pericentriolar proteins are not recruited to the sperm centrioles until after completion of meiosis. Depletion of kinesin-1 heavy chain or its binding partner resulted in premature centrosome maturation during meiosis and growth of a sperm aster that could capture the oocyte meiotic spindle. Kinesin prevents recruitment of pericentriolar proteins by coating the sperm DNA and centrioles and thus prevents triploidy by a nonmotor mechanism. |
| Volume | 22(4) |
| Pages | 788-98 |
| Published | 2012-4-17 |
| DOI | 10.1016/j.devcel.2012.01.010 |
| PII | S1534-5807(12)00044-5 |
| PMID | 22465668 |
| PMC | PMC3606814 |
| MeSH | Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins Cell Cycle Proteins Cells, Cultured Centrioles / metabolism* Centrosome / physiology* Embryo, Nonmammalian Female Fertilization Fluorescent Antibody Technique Kinesins / antagonists & inhibitors Kinesins / genetics Kinesins / metabolism* Male Meiosis / physiology* Oocytes / cytology Oocytes / physiology* Oogenesis / physiology Spermatozoa / cytology Spermatozoa / physiology* Spindle Apparatus / physiology |
| IF | 10.092 |
| Times Cited | 14 |
| WOS Category | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1489 tm1145 |