| RRC ID |
46412
|
| 著者 |
Monteiro MI, Ahlawat S, Kowalski JR, Malkin E, Koushika SP, Juo P.
|
| タイトル |
The kinesin-3 family motor KLP-4 regulates anterograde trafficking of GLR-1 glutamate receptors in the ventral nerve cord of Caenorhabditis elegans.
|
| ジャーナル |
Mol Biol Cell
|
| Abstract |
The transport of glutamate receptors from the cell body to synapses is essential during neuronal development and may contribute to the regulation of synaptic strength in the mature nervous system. We previously showed that cyclin-dependent kinase-5 (CDK-5) positively regulates the abundance of GLR-1 glutamate receptors at synapses in the ventral nerve cord (VNC) of Caenorhabditis elegans. Here we identify a kinesin-3 family motor klp-4/KIF13 in a cdk-5 suppressor screen for genes that regulate GLR-1 trafficking. klp-4 mutants have decreased abundance of GLR-1 in the VNC. Genetic analysis of klp-4 and the clathrin adaptin unc-11/AP180 suggests that klp-4 functions before endocytosis in the ventral cord. Time-lapse microscopy indicates that klp-4 mutants exhibit decreased anterograde flux of GLR-1. Genetic analysis of cdk-5 and klp-4 suggests that they function in the same pathway to regulate GLR-1 in the VNC. Interestingly, GLR-1 accumulates in cell bodies of cdk-5 but not klp-4 mutants. However, GLR-1 does accumulate in klp-4-mutant cell bodies if receptor degradation in the multivesicular body/lysosome pathway is blocked. This study identifies kinesin KLP-4 as a novel regulator of anterograde glutamate receptor trafficking and reveals a cellular control mechanism by which receptor cargo is targeted for degradation in the absence of its motor.
|
| 巻・号 |
23(18)
|
| ページ |
3647-62
|
| 公開日 |
2012-9-1
|
| DOI |
10.1091/mbc.E12-04-0334
|
| PII |
mbc.E12-04-0334
|
| PMID |
22855524
|
| PMC |
PMC3442412
|
| MeSH |
Amino Acid Sequence
Animals
Animals, Genetically Modified
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Cyclin-Dependent Kinase 5 / genetics
Cyclin-Dependent Kinase 5 / metabolism
Endocytosis
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Interneurons / metabolism
Kinesins / genetics
Kinesins / metabolism*
Lysosomes / metabolism
Microscopy, Fluorescence
Molecular Sequence Data
Multivesicular Bodies / metabolism
Mutation
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Nervous System / cytology
Nervous System / metabolism*
Protein Transport
Receptors, AMPA / genetics
Receptors, AMPA / metabolism*
Sequence Homology, Amino Acid
Signal Transduction
Synapses / metabolism
Time-Lapse Imaging
|
| IF |
3.791
|
| 引用数 |
17
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2114 |
