Reference - Detail
| RRC ID | 46431 |
|---|---|
| Author | Rosains J, Mango SE. |
| Title | Genetic characterization of smg-8 mutants reveals no role in C. elegans nonsense mediated decay. |
| Journal | PLoS One |
| Abstract |
The nonsense mediated decay (NMD) pathway degrades mRNAs bearing premature translation termination codons. In mammals, SMG-8 has been implicated in the NMD pathway, in part by its association with SMG-1 kinase. Here we use four independent assays to show that C. elegans smg-8 is not required to degrade nonsense-containing mRNAs. We examine the genetic requirement for smg-8 to destabilize the endogenous, natural NMD targets produced by alternative splicing of rpl-7a and rpl-12. We test smg-8 for degradation of the endogenous, NMD target generated by unc-54(r293), which lacks a normal polyadenylation site. We probe the effect of smg-8 on the exogenous NMD target produced by myo-3::GFP, which carries a long 3' untranslated region that destabilizes mRNAs. None of these known NMD targets is influenced by smg-8 mutations. In addition, smg-8 animals lack classical Smg mutant phenotypes such as a reduced brood size or abnormal vulva. We conclude that smg-8 is unlikely to encode a component critical for NMD. |
| Volume | 7(11) |
| Pages | e49490 |
| Published | 2012-1-1 |
| DOI | 10.1371/journal.pone.0049490 |
| PII | PONE-D-12-16297 |
| PMID | 23166684 |
| PMC | PMC3500306 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Gene Expression Regulation Gene Order Genes, Reporter Mutation* Nonsense Mediated mRNA Decay* Phenotype RNA, Messenger / genetics RNA, Messenger / metabolism Ribosomal Proteins / genetics Ribosomal Proteins / metabolism |
| IF | 2.74 |
| Times Cited | 5 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2937 tm1917 |