Reference - Detail
| RRC ID | 46451 |
|---|---|
| Author | Studencka M, Wesołowski R, Opitz L, Salinas-Riester G, Wisniewski JR, Jedrusik-Bode M. |
| Title | Transcriptional repression of Hox genes by C. elegans HP1/HPL and H1/HIS-24. |
| Journal | PLoS Genet |
| Abstract |
Elucidation of the biological role of linker histone (H1) and heterochromatin protein 1 (HP1) in mammals has been difficult owing to the existence of a least 11 distinct H1 and three HP1 subtypes in mice. Caenorhabditis elegans possesses two HP1 homologues (HPL-1 and HPL-2) and eight H1 variants. Remarkably, one of eight H1 variants, HIS-24, is important for C. elegans development. Therefore we decided to analyse in parallel the transcriptional profiles of HIS-24, HPL-1/-2 deficient animals, and their phenotype, since hpl-1, hpl-2, and his-24 deficient nematodes are viable. Global transcriptional analysis of the double and triple mutants revealed that HPL proteins and HIS-24 play gene-specific roles, rather than a general repressive function. We showed that HIS-24 acts synergistically with HPL to allow normal reproduction, somatic gonad development, and vulval cell fate decision. Furthermore, the hpl-2; his-24 double mutant animals displayed abnormal development of the male tail and ectopic expression of C. elegans HOM-C/Hox genes (egl-5 and mab-5), which are involved in the developmental patterning of male mating structures. We found that HPL-2 and the methylated form of HIS-24 specifically interact with the histone H3 K27 region in the trimethylated state, and HIS-24 associates with the egl-5 and mab-5 genes. Our results establish the interplay between HPL-1/-2 and HIS-24 proteins in the regulation of positional identity in C. elegans males. |
| Volume | 8(9) |
| Pages | e1002940 |
| Published | 2012-9-1 |
| DOI | 10.1371/journal.pgen.1002940 |
| PII | PGENETICS-D-12-00349 |
| PMID | 23028351 |
| PMC | PMC3441639 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Chromosomal Proteins, Non-Histone* / deficiency Chromosomal Proteins, Non-Histone* / genetics Female Gene Expression Regulation, Developmental Histone-Lysine N-Methyltransferase / genetics Histone-Lysine N-Methyltransferase / metabolism Histones / deficiency Histones / genetics* Histones / metabolism Homeodomain Proteins / metabolism Male Methylation Mutation Tail / growth & development Transcription Factors / metabolism Vulva / cytology Vulva / growth & development |
| IF | 5.175 |
| Times Cited | 18 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1489 tm1624 |