論文 - 詳細
| RRC ID | 46495 |
|---|---|
| 著者 | Seidel HS, Ailion M, Li J, van Oudenaarden A, Rockman MV, Kruglyak L. |
| タイトル | A novel sperm-delivered toxin causes late-stage embryo lethality and transmission ratio distortion in C. elegans. |
| ジャーナル | PLoS Biol |
| Abstract |
The evolutionary fate of an allele ordinarily depends on its contribution to host fitness. Occasionally, however, genetic elements arise that are able to gain a transmission advantage while simultaneously imposing a fitness cost on their hosts. We previously discovered one such element in C. elegans that gains a transmission advantage through a combination of paternal-effect killing and zygotic self-rescue. Here we demonstrate that this element is composed of a sperm-delivered toxin, peel-1, and an embryo-expressed antidote, zeel-1. peel-1 and zeel-1 are located adjacent to one another in the genome and co-occur in an insertion/deletion polymorphism. peel-1 encodes a novel four-pass transmembrane protein that is expressed in sperm and delivered to the embryo via specialized, sperm-specific vesicles. In the absence of zeel-1, sperm-delivered PEEL-1 causes lethal defects in muscle and epidermal tissue at the 2-fold stage of embryogenesis. zeel-1 is expressed transiently in the embryo and encodes a novel six-pass transmembrane domain fused to a domain with sequence similarity to zyg-11, a substrate-recognition subunit of an E3 ubiquitin ligase. zeel-1 appears to have arisen recently, during an expansion of the zyg-11 family, and the transmembrane domain of zeel-1 is required and partially sufficient for antidote activity. Although PEEL-1 and ZEEL-1 normally function in embryos, these proteins can act at other stages as well. When expressed ectopically in adults, PEEL-1 kills a variety of cell types, and ectopic expression of ZEEL-1 rescues these effects. Our results demonstrate that the tight physical linkage between two novel transmembrane proteins has facilitated their co-evolution into an element capable of promoting its own transmission to the detriment of organisms carrying it. |
| 巻・号 | 9(7) |
| ページ | e1001115 |
| 公開日 | 2011-7-1 |
| DOI | 10.1371/journal.pbio.1001115 |
| PII | PBIOLOGY-D-10-00587 |
| PMID | 21814493 |
| PMC | PMC3144186 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Embryonic Development Epidermis / abnormalities Female Gene Dosage Genes, Lethal Genetic Linkage Heredity Male Membrane Proteins / genetics* Membrane Proteins / metabolism Molecular Sequence Data Muscles / abnormalities Organelles / metabolism Phenotype Phylogeny Protein Structure, Tertiary Spermatozoa / metabolism* Toxins, Biological / genetics* Toxins, Biological / metabolism |
| IF | 7.076 |
| 引用数 | 80 |
| WOS 分野 | BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 線虫 | tm3419 |