論文 - 詳細
| RRC ID | 46547 |
|---|---|
| 著者 | Zhang D, Isack NR, Glodowski DR, Liu J, Chen CC, Xu XZ, Grant BD, Rongo C. |
| タイトル | RAB-6.2 and the retromer regulate glutamate receptor recycling through a retrograde pathway. |
| ジャーナル | J Cell Biol |
| Abstract |
Regulated membrane trafficking of AMPA-type glutamate receptors (AMPARs) is a key mechanism underlying synaptic plasticity, yet the pathways used by AMPARs are not well understood. In this paper, we show that the AMPAR subunit GLR-1 in Caenorhabditis elegans utilizes the retrograde transport pathway to regulate AMPAR synaptic abundance. Mutants for rab-6.2, the retromer genes vps-35 and snx-1, and rme-8 failed to recycle GLR-1 receptors, resulting in GLR-1 turnover and behavioral defects indicative of diminished GLR-1 function. In contrast, expression of constitutively active RAB-6.2 drove the retrograde transport of GLR-1 from dendrites back to cell body Golgi. We also find that activated RAB-6.2 bound to and colocalized with the PDZ/phosphotyrosine binding domain protein LIN-10. RAB-6.2 recruited LIN-10. Moreover, the regulation of GLR-1 transport by RAB-6.2 required LIN-10 activity. Our results demonstrate a novel role for RAB-6.2, its effector LIN-10, and the retromer complex in maintaining synaptic strength by recycling AMPARs along the retrograde transport pathway. |
| 巻・号 | 196(1) |
| ページ | 85-101 |
| 公開日 | 2012-1-9 |
| DOI | 10.1083/jcb.201104141 |
| PII | jcb.201104141 |
| PMID | 22213799 |
| PMC | PMC3255976 |
| MeSH | Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology Endocytosis Endosomes / metabolism Golgi Apparatus / metabolism Membrane Proteins / analysis Membrane Proteins / metabolism Membrane Proteins / physiology Protein Transport Receptors, AMPA / metabolism* rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism rab GTP-Binding Proteins / physiology* |
| IF | 8.811 |
| 引用数 | 31 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm847 |