Reference - Detail
|Author||Kovacevic I, Cram EJ.|
|Title||FLN-1/filamin is required for maintenance of actin and exit of fertilized oocytes from the spermatheca in C. elegans.|
Filamin, known primarily for its actin cross-linking function, is a stretch-sensitive structural and signaling scaffold that binds transmembrane receptors and a wide variety of intracellular signaling proteins. The Caenorhabditis elegans filamin ortholog, FLN-1, has a well conserved overall structure, including an N-terminal actin-binding domain, and a series of 20 immunoglobulin (Ig)-like repeats. FLN-1 partially colocalizes with actin filaments in spermathecal and uterine cells. Analysis of phenotypes resulting from a deletion allele and RNAi depletion indicates FLN-1 is required to maintain the actin cytoskeleton in the spermatheca and uterus, and to allow the exit of embryos from the spermatheca. FLN-1 deficient animals accumulate embryos in the spermatheca, lay damaged and unfertilized eggs, and consequently exhibit dramatically reduced brood sizes. The phospholipase PLC-1 is also required for the exit of embryos from the spermatheca, and analysis of doubly mutant animals suggests that PLC-1 and FLN-1 act in the same pathway to promote proper transit of embryos from the spermatheca to the uterus. Given the modular protein structure, subcellular localization, genetic interaction with PLC-1, and known mechanosensory functions of filamin, we postulate that FLN-1 may be required to convert mechanical information about the presence of the oocyte into a biochemical signal, thereby allowing timely exit of the embryo from the spermatheca.
|MeSH||Actins / metabolism* Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Contractile Proteins / antagonists & inhibitors Contractile Proteins / chemistry Contractile Proteins / genetics Contractile Proteins / physiology* Female Fertility / genetics Fertility / physiology Filamins / antagonists & inhibitors Filamins / chemistry Filamins / genetics Filamins / physiology* Genitalia / growth & development Genitalia / physiology Male Microfilament Proteins / antagonists & inhibitors Microfilament Proteins / chemistry Microfilament Proteins / genetics Microfilament Proteins / physiology* Oocytes / physiology* Protein Isoforms / chemistry Protein Isoforms / genetics Protein Isoforms / physiology RNA Interference Signal Transduction / physiology|
|WOS Category||DEVELOPMENTAL BIOLOGY|