論文 - 詳細
| RRC ID | 46635 |
|---|---|
| 著者 | Li Y, Wei Q, Zhang Y, Ling K, Hu J. |
| タイトル | The small GTPases ARL-13 and ARL-3 coordinate intraflagellar transport and ciliogenesis. |
| ジャーナル | J Cell Biol |
| Abstract |
Intraflagellar transport (IFT) machinery mediates the bidirectional movement of cargos that are required for the assembly and maintenance of cilia. However, little is known about how IFT is regulated in vivo. In this study, we show that the small guanosine triphosphatase (GTPase) adenosine diphosphate ribosylation factor-like protein 13 (ARL-13) encoded by the Caenorhabditis elegans homologue of the human Joubert syndrome causal gene ARL13B, localizes exclusively to the doublet segment of the cilium. arl-13 mutants have shortened cilia with various ultrastructural deformities and a disrupted association between IFT subcomplexes A and B. Intriguingly, depletion of ARL-3, another ciliary small GTPase, partially suppresses ciliogenesis defects in arl-13 mutants by indirectly restoring binding between IFT subcomplexes A and B. Rescue of arl-13 mutants by ARL-3 depletion is mediated by an HDAC6 deacetylase-dependent pathway. Thus, we propose that two conserved small GTPases, ARL-13 and ARL-3, coordinate to regulate IFT and that perturbing this balance results in cilia deformation. |
| 巻・号 | 189(6) |
| ページ | 1039-51 |
| 公開日 | 2010-6-14 |
| DOI | 10.1083/jcb.200912001 |
| PII | jcb.200912001 |
| PMID | 20530210 |
| PMC | PMC2886347 |
| MeSH | Animals Animals, Genetically Modified Biological Transport / physiology Caenorhabditis elegans / cytology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cilia / physiology* Cilia / ultrastructure Flagella / metabolism* Flagella / ultrastructure Histone Deacetylases / genetics Histone Deacetylases / metabolism Humans Kinesins / metabolism Monomeric GTP-Binding Proteins / genetics Monomeric GTP-Binding Proteins / metabolism* Mutation Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism |
| IF | 8.811 |
| 引用数 | 89 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2322 tm1703 |