RRC ID 46635
Author Li Y, Wei Q, Zhang Y, Ling K, Hu J.
Title The small GTPases ARL-13 and ARL-3 coordinate intraflagellar transport and ciliogenesis.
Journal J Cell Biol
Abstract Intraflagellar transport (IFT) machinery mediates the bidirectional movement of cargos that are required for the assembly and maintenance of cilia. However, little is known about how IFT is regulated in vivo. In this study, we show that the small guanosine triphosphatase (GTPase) adenosine diphosphate ribosylation factor-like protein 13 (ARL-13) encoded by the Caenorhabditis elegans homologue of the human Joubert syndrome causal gene ARL13B, localizes exclusively to the doublet segment of the cilium. arl-13 mutants have shortened cilia with various ultrastructural deformities and a disrupted association between IFT subcomplexes A and B. Intriguingly, depletion of ARL-3, another ciliary small GTPase, partially suppresses ciliogenesis defects in arl-13 mutants by indirectly restoring binding between IFT subcomplexes A and B. Rescue of arl-13 mutants by ARL-3 depletion is mediated by an HDAC6 deacetylase-dependent pathway. Thus, we propose that two conserved small GTPases, ARL-13 and ARL-3, coordinate to regulate IFT and that perturbing this balance results in cilia deformation.
Volume 189(6)
Pages 1039-51
Published 2010-6-14
DOI 10.1083/jcb.200912001
PII jcb.200912001
PMID 20530210
PMC PMC2886347
MeSH Animals Animals, Genetically Modified Biological Transport / physiology Caenorhabditis elegans / cytology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cilia / physiology* Cilia / ultrastructure Flagella / metabolism* Flagella / ultrastructure Histone Deacetylases / genetics Histone Deacetylases / metabolism Humans Kinesins / metabolism Monomeric GTP-Binding Proteins / genetics Monomeric GTP-Binding Proteins / metabolism* Mutation Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism
IF 8.811
Times Cited 89
C.elegans tm2322 tm1703