RRC ID 46686
著者 Tarailo-Graovac M, Wang J, Chu JS, Tu D, Baillie DL, Chen N.
タイトル Spindle assembly checkpoint genes reveal distinct as well as overlapping expression that implicates MDF-2/Mad2 in postembryonic seam cell proliferation in Caenorhabditis elegans.
ジャーナル BMC Cell Biol
Abstract BACKGROUND:The spindle assembly checkpoint (SAC) delays anaphase onset by inhibiting the activity of the anaphase promoting complex/cyclosome (APC/C) until all of the kinetochores have properly attached to the spindle. The importance of SAC genes for genome stability is well established; however, the roles these genes play, during postembryonic development of a multicellular organism, remain largely unexplored.
RESULTS:We have used GFP fusions of 5' upstream intergenic regulatory sequences to assay spatiotemporal expression patterns of eight conserved genes implicated in the spindle assembly checkpoint function in Caenorhabditis elegans. We have shown that regulatory sequences for all of the SAC genes drive ubiquitous GFP expression during early embryonic development. However, postembryonic spatial analysis revealed distinct, tissue-specific expression of SAC genes with striking co-expression in seam cells, as well as in the gut. Additionally, we show that the absence of MDF-2/Mad2 (one of the checkpoint genes) leads to aberrant number and alignment of seam cell nuclei, defects mainly attributed to abnormal postembryonic cell proliferation. Furthermore, we show that these defects are completely rescued by fzy-1(h1983)/CDC20, suggesting that regulation of the APC/CCDC20 by the SAC component MDF-2 is important for proper postembryonic cell proliferation.
CONCLUSION:Our results indicate that SAC genes display different tissue-specific expression patterns during postembryonic development in C. elegans with significant co-expression in hypodermal seam cells and gut cells, suggesting that these genes have distinct as well as overlapping roles in postembryonic development that may or may not be related to their established roles in mitosis. Furthermore, we provide evidence, by monitoring seam cell lineage, that one of the checkpoint genes is required for proper postembryonic cell proliferation. Importantly, our research provides the first evidence that postembryonic cell division is more sensitive to SAC loss, in particular MDF-2 loss, than embryonic cell division.
巻・号 11
ページ 71
公開日 2010-9-21
DOI 10.1186/1471-2121-11-71
PII 1471-2121-11-71
PMID 20858267
PMC PMC2955571
MeSH 5' Untranslated Regions / genetics Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cdc20 Proteins Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Proliferation Embryonic Development / genetics Gene Expression Profiling Genes, cdc / physiology* Genetic Engineering Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Morphogenesis / genetics Mutation / genetics Protein Binding Spindle Apparatus / genetics Spindle Apparatus / metabolism*
IF 3.066
引用数 2
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm2190