RRC ID 46708
著者 Nakae I, Fujino T, Kobayashi T, Sasaki A, Kikko Y, Fukuyama M, Gengyo-Ando K, Mitani S, Kontani K, Katada T.
タイトル The arf-like GTPase Arl8 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans.
ジャーナル Mol Biol Cell
Abstract Late endocytic organelles including lysosomes are highly dynamic acidic organelles. Late endosomes and lysosomes directly fuse for content mixing to form hybrid organelles, from which lysosomes are reformed. It is not fully understood how these processes are regulated and maintained. Here we show that the Caenorhabditis elegans ARL-8 GTPase is localized primarily to lysosomes and involved in late endosome-lysosome fusion in the macrophage-like coelomocytes. Loss of arl-8 results in an increase in the number of late endosomal/lysosomal compartments, which are smaller than wild type. In arl-8 mutants, late endosomal compartments containing endocytosed macromolecules fail to fuse with lysosomal compartments enriched in the aspartic protease ASP-1. Furthermore, loss of arl-8 strongly suppresses formation of enlarged late endosome-lysosome hybrid organelles caused by mutations of cup-5, which is the orthologue of human mucolipin-1. These findings suggest that ARL-8 mediates delivery of endocytosed macromolecules to lysosomes by facilitating late endosome-lysosome fusion.
巻・号 21(14)
ページ 2434-42
公開日 2010-7-15
DOI 10.1091/mbc.e09-12-1010
PII E09-12-1010
PMID 20484575
PMC PMC2903672
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology Caenorhabditis elegans / enzymology* Caenorhabditis elegans Proteins / metabolism* Cell Compartmentation Embryonic Development Endocytosis* Endosomes / enzymology GTP Phosphohydrolases / metabolism* Intracellular Membranes / enzymology Lysosomes / metabolism* Mutation / genetics Protein Transport Serum Albumin, Bovine / metabolism Subcellular Fractions / metabolism Time Factors
IF 3.791
引用数 42
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm2504